Movement of HCs on HA- or VN-coated plastic surfaces
| Surface and treatment . | n* . | Speed of moving cells (μm/min) . | % . |
|---|---|---|---|
| HA alone | 12 | 7.6 ± 0.4 | 39 ± 3 |
| HA + IL-8 | 12 | 3.5 ± 0.2 | 9 ± 1 |
| HA + IL-8 + anti-IL-8 MoAb | 3 | 8.7 ± 1.2 | 35 ± 4 |
| HA + IgG1 + IL-8 | 3 | 3.2 ± 0.2 | 10 ± 1 |
| HA + MIP-1α | 4 | 6.6 ± 0.5 | 38 ± 3 |
| VN alone | 6 | 3.9 ± 1.6 | 8 ± 2 |
| VN + IL-8 | 6 | 6.4 ± 1.2 | 22 ± 3 |
| Surface and treatment . | n* . | Speed of moving cells (μm/min) . | % . |
|---|---|---|---|
| HA alone | 12 | 7.6 ± 0.4 | 39 ± 3 |
| HA + IL-8 | 12 | 3.5 ± 0.2 | 9 ± 1 |
| HA + IL-8 + anti-IL-8 MoAb | 3 | 8.7 ± 1.2 | 35 ± 4 |
| HA + IgG1 + IL-8 | 3 | 3.2 ± 0.2 | 10 ± 1 |
| HA + MIP-1α | 4 | 6.6 ± 0.5 | 38 ± 3 |
| VN alone | 6 | 3.9 ± 1.6 | 8 ± 2 |
| VN + IL-8 | 6 | 6.4 ± 1.2 | 22 ± 3 |
Movement was recorded by time-lapse video microscopy (TLVM) over 2 hours. Results are means ± 1 SEM. HC indicates hairy cell; HA, hyaluronan; VN, vitronectin; IL, interleukin; MoAb, monoclonal antibody; MIP-1α, macrophage inflammatory protein-1α.
Number of separate experiments carried out; where n ≤ 4, the experiments involved different HCL patients; where n = 6 or 12, 6 different patients were studied.