Reconstitution in secondary recipients from primary 38+34− donor cells
| Donor cells . | Months after BMT . | No. of mice . | % Ly5.1+ . | T and B lymphocytes . | Macrophages (M) and granulocytes (G) . |
|---|---|---|---|---|---|
| WBM from 1st recipients (1 × 106) | 12 | 7 | 31 ± 5 | T: 33.5 ± 2.9 | M: 39 ± 3 |
| B: 44.8 ± 4.5 | G: 35.2 ± 2.9 | ||||
| Sorted Ly5.1 cells from 1st recipients (1.4 × 106) | 7 | 7 | 79 ± 5 | T: 70.3 ± 8.2 | M: 63.3 ± 6 |
| B: 91.3 ± 4.3 | G: 55.1 ± 6 | ||||
| Sorted Ly5.1 cells from 1st recipients (1 × 106) | 8 | 6 | 40 ± 8 | 47.5 ± 8.2 | 49.7 ± 36.6 |
| Donor cells . | Months after BMT . | No. of mice . | % Ly5.1+ . | T and B lymphocytes . | Macrophages (M) and granulocytes (G) . |
|---|---|---|---|---|---|
| WBM from 1st recipients (1 × 106) | 12 | 7 | 31 ± 5 | T: 33.5 ± 2.9 | M: 39 ± 3 |
| B: 44.8 ± 4.5 | G: 35.2 ± 2.9 | ||||
| Sorted Ly5.1 cells from 1st recipients (1.4 × 106) | 7 | 7 | 79 ± 5 | T: 70.3 ± 8.2 | M: 63.3 ± 6 |
| B: 91.3 ± 4.3 | G: 55.1 ± 6 | ||||
| Sorted Ly5.1 cells from 1st recipients (1 × 106) | 8 | 6 | 40 ± 8 | 47.5 ± 8.2 | 49.7 ± 36.6 |
Twelve months after primary transplantation of 100 Ly5.1 38+34− donor cells, WBM or Ly5.1-marker-sorted BM cells from the Ly5.2 primary recipient mice were infused into lethally irradiated Ly5.2 secondary recipients. PB was collected at the times indicated, and the total percentage of Ly5.1 donor-cell engraftment, as well as the percentage of Ly5.1 donor-cell engraftment in the different lineages, was determined by FACS analysis. The level of Ly5.1-positive cells in the WBM from the first recipients (first row of table) was 51.6%. Plus-minus values are mean ± SE percentages.