Effect of expression of inactive mutant HRI on the growth of MEL cells
| Cell lines . | Cell number (×106) . | ||||
|---|---|---|---|---|---|
| DMSO, day 4 . | DMSO, day 5 . | ||||
| − . | + . | − . | + . | % . | |
| Uninfected | 63.5 | 65.0 | 90.5 | 71.5 | 100 |
| Vector control | 67.2 | 80.0 | 3-150 | 74.0 | 103.5 |
| K199R HRI | 60.8 | 105.0 | 78.0 | 123.0 | 172.0 |
| Δ10 HRI | 72.0 | 83.0 | 77.0 | 98.8 | 138.2 |
| Bcl-2 | 70.5 | 59.5 | 82.5 | 74.5 | 104.2 |
| Cell lines . | Cell number (×106) . | ||||
|---|---|---|---|---|---|
| DMSO, day 4 . | DMSO, day 5 . | ||||
| − . | + . | − . | + . | % . | |
| Uninfected | 63.5 | 65.0 | 90.5 | 71.5 | 100 |
| Vector control | 67.2 | 80.0 | 3-150 | 74.0 | 103.5 |
| K199R HRI | 60.8 | 105.0 | 78.0 | 123.0 | 172.0 |
| Δ10 HRI | 72.0 | 83.0 | 77.0 | 98.8 | 138.2 |
| Bcl-2 | 70.5 | 59.5 | 82.5 | 74.5 | 104.2 |
MEL cells with expression vector, K199R HRI, Δ10 HRI, and Bcl-2, and the uninfected MEL cells were cultured and induced for erythroid differentiation as described in the Figure 3 legend. At days 4 and 5, both control and induced cells were harvested and counted. The percentage indicates the increase in cell number upon expression of inactive mutant HRI, Bcl-2, or vector control after DMSO inductin at day 5. The cell number of uninfected cells at day 5 is defined as 100%.
Cell number was not determined.