Effect of expression of mutant HRI on the cell-cycle status in MEL cells
| Cell lines . | Without DMSO . | With DMSO . | ||
|---|---|---|---|---|
| G0/G1 . | S + G2/M . | G0/G1 . | S + G2/M . | |
| Uninfected | 76.51 | 23.49 | 93.88 | 6.12 |
| Vector | 78.44 | 21.56 | 91.58 | 8.42 |
| K199R HRI | 80.37 | 19.63 | 84.37 | 15.63 |
| Δ10 HRI | 72.62 | 27.38 | 87.56 | 12.44 |
| Δ20 HRI | 63.39 | 36.61 | 90.46 | 9.54 |
| Cell lines . | Without DMSO . | With DMSO . | ||
|---|---|---|---|---|
| G0/G1 . | S + G2/M . | G0/G1 . | S + G2/M . | |
| Uninfected | 76.51 | 23.49 | 93.88 | 6.12 |
| Vector | 78.44 | 21.56 | 91.58 | 8.42 |
| K199R HRI | 80.37 | 19.63 | 84.37 | 15.63 |
| Δ10 HRI | 72.62 | 27.38 | 87.56 | 12.44 |
| Δ20 HRI | 63.39 | 36.61 | 90.46 | 9.54 |
MEL cells expressing vector and HRI as indicated were cultured and induced for erythroid differentiation as described in the Figure 5legend. At day 4.5, both control and induced cells were harvested and stained with propidium iodide for cell-cycle analysis as described previously.53 The number represents the percentage of cells in each phase of cell cycle.