In vitro growth of marrow mesenchymal progenitors
| Case no. . | Assay time, months post-SCT . | CFU-F, per 1 × 106 MNCs . | Marrow stromal layers . | ||
|---|---|---|---|---|---|
| Type of growth . | Confluence, week . | Adipogenesis, week . | |||
| 1 | 2 | ND | confluent3-150 | 5 | 2 |
| 6 | 30 | confluent | 5 | 2 | |
| 2 | 2 | 21 | subconfluent3-151 | 5 | 4 |
| 3 | 50 | 7 | confluent | 5 | 3 |
| 62 | ND | confluent | 4 | 3 | |
| 4 | 3 | 10 | subconfluent | 5 | 4 |
| 5 | 44 | 0 | no growth | — | — |
| 59 | 7 | confluent | 4 | 3 | |
| 6 | 2 | ND | subconfluent | 4 | 3 |
| 7 | 3 | 0 | no growth | — | — |
| 6 | 3 | defective3-152 | NR | — | |
| 9 | 20 | confluent | 4 | 2 | |
| 8 | 48 | 0 | no growth | — | — |
| 79 | 10 | confluent | 5 | 4 | |
| 9 | 2 | 20 | confluent | 5 | 4 |
| 10 | 3 | 0 | no growth | — | — |
| 6 | 0 | no growth | — | — | |
| 10 | 0 | no growth | — | — | |
| 18 | 3 | defective | NR | — | |
| 31 | 16 | subconfluent | 4 | 3 | |
| 11 | 1 | ND | subconfluent | 5 | 4 |
| 12 | 82 | ND | subconfluent | 5 | 4 |
| 13 | 3 | 0 | no growth | — | — |
| 6 | 2 | defective | NR | — | |
| 17 | 12 | subconfluent | 4 | 3 | |
| 14 | 17 | 18 | confluent | 4 | 3 |
| Normal controls (n = 55) | |||||
| Mean ± SEM | 56 ± 6 | confluent | 4 ± 1 | 3 ± 0.4 | |
| Case no. . | Assay time, months post-SCT . | CFU-F, per 1 × 106 MNCs . | Marrow stromal layers . | ||
|---|---|---|---|---|---|
| Type of growth . | Confluence, week . | Adipogenesis, week . | |||
| 1 | 2 | ND | confluent3-150 | 5 | 2 |
| 6 | 30 | confluent | 5 | 2 | |
| 2 | 2 | 21 | subconfluent3-151 | 5 | 4 |
| 3 | 50 | 7 | confluent | 5 | 3 |
| 62 | ND | confluent | 4 | 3 | |
| 4 | 3 | 10 | subconfluent | 5 | 4 |
| 5 | 44 | 0 | no growth | — | — |
| 59 | 7 | confluent | 4 | 3 | |
| 6 | 2 | ND | subconfluent | 4 | 3 |
| 7 | 3 | 0 | no growth | — | — |
| 6 | 3 | defective3-152 | NR | — | |
| 9 | 20 | confluent | 4 | 2 | |
| 8 | 48 | 0 | no growth | — | — |
| 79 | 10 | confluent | 5 | 4 | |
| 9 | 2 | 20 | confluent | 5 | 4 |
| 10 | 3 | 0 | no growth | — | — |
| 6 | 0 | no growth | — | — | |
| 10 | 0 | no growth | — | — | |
| 18 | 3 | defective | NR | — | |
| 31 | 16 | subconfluent | 4 | 3 | |
| 11 | 1 | ND | subconfluent | 5 | 4 |
| 12 | 82 | ND | subconfluent | 5 | 4 |
| 13 | 3 | 0 | no growth | — | — |
| 6 | 2 | defective | NR | — | |
| 17 | 12 | subconfluent | 4 | 3 | |
| 14 | 17 | 18 | confluent | 4 | 3 |
| Normal controls (n = 55) | |||||
| Mean ± SEM | 56 ± 6 | confluent | 4 ± 1 | 3 ± 0.4 | |