Table 6. Megakaryocytic colony formation... | American Society of Hematology

Table 6.

Megakaryocytic colony formation in the absence of added PEG-rHu MGDF by progenitor cells from patients with ET and healthy subjects

Experiment no.	No. of cells plated	Culture volume	No. of samples per group	Healthy	Mean no. of megakaryocytic colonies ± SEM
Experiment no.	No. of cells plated	Culture volume	No. of samples per group	Healthy	ET^6-150	P^6-150,^6-151	Difference^6-150
155/159	1.5 × 10⁵	0.7	4	0.4 ± 0.4	11.5 ± 2.1	.001 < P < .01	11.1
160	1.5 × 10⁵	0.7	4	6.1 ± 1.0	20.5 ± 1.4	< .001	14.4
161	1.5 × 10⁵	0.7	4	0.3 ± 0.3	5.8 ± 0.8	< .001	5.5
164	1.5 × 10⁵	0.7	3	4.0 ± 0.58	5.0 ± 0.58	.3 < P < .4	1.0
169	1.5 × 10⁵	0.7	3	14.3 ± 2.6	28.7 ± 1.76	.01	14.4
170	1.5 × 10⁵	0.7	3	16.3 ± 2.0	16.7 ± 1.76	.9	0.4
175	1.5 × 10⁵	0.7	3	14.0 ± 1.5	16.3 ± 0.88	.2 < P < .3	2.3
209	4 × 10⁴	0.1	5	0.2 ± 0.2	1.2 ± 0.37	.05	1.0
210	4 × 10⁴	0.1	5	2.0 ± 0.3	3.2 ± 0.2	.01	1.2
212	4 × 10⁴	0.1	5	0.6 ± 0.25	1.6 ± 0.25	.02	1.0
217	4 × 10⁴	0.1	5	1.2 ± 0.2	2.6 ± 0.25	.001 < P < .01	1.4
218	4 × 10⁴	0.1	5	3.6 ± 0.25	4.0 ± 0.55	.5	0.4
221	4 × 10⁴	0.1	5	2.8 ± 0.37	8.2 ± 0.58	< .001	5.4
225	4 × 10⁴	0.1	5	2.0 ± 0.3	2.2 ± 0.5	≃ .7	0.2
227	4 × 10⁴	0.1	5	1.8 ± 0.37	3.8 ± 0.37	< .01	2.0
228	4 × 10⁴	0.1	5	0.0	2.0 ± 0.3	< .001	2.0

Experiment no.	No. of cells plated	Culture volume	No. of samples per group	Healthy	Mean no. of megakaryocytic colonies ± SEM
Experiment no.	No. of cells plated	Culture volume	No. of samples per group	Healthy	ET^6-150	P^6-150,^6-151	Difference^6-150
155/159	1.5 × 10⁵	0.7	4	0.4 ± 0.4	11.5 ± 2.1	.001 < P < .01	11.1
160	1.5 × 10⁵	0.7	4	6.1 ± 1.0	20.5 ± 1.4	< .001	14.4
161	1.5 × 10⁵	0.7	4	0.3 ± 0.3	5.8 ± 0.8	< .001	5.5
164	1.5 × 10⁵	0.7	3	4.0 ± 0.58	5.0 ± 0.58	.3 < P < .4	1.0
169	1.5 × 10⁵	0.7	3	14.3 ± 2.6	28.7 ± 1.76	.01	14.4
170	1.5 × 10⁵	0.7	3	16.3 ± 2.0	16.7 ± 1.76	.9	0.4
175	1.5 × 10⁵	0.7	3	14.0 ± 1.5	16.3 ± 0.88	.2 < P < .3	2.3
209	4 × 10⁴	0.1	5	0.2 ± 0.2	1.2 ± 0.37	.05	1.0
210	4 × 10⁴	0.1	5	2.0 ± 0.3	3.2 ± 0.2	.01	1.2
212	4 × 10⁴	0.1	5	0.6 ± 0.25	1.6 ± 0.25	.02	1.0
217	4 × 10⁴	0.1	5	1.2 ± 0.2	2.6 ± 0.25	.001 < P < .01	1.4
218	4 × 10⁴	0.1	5	3.6 ± 0.25	4.0 ± 0.55	.5	0.4
221	4 × 10⁴	0.1	5	2.8 ± 0.37	8.2 ± 0.58	< .001	5.4
225	4 × 10⁴	0.1	5	2.0 ± 0.3	2.2 ± 0.5	≃ .7	0.2
227	4 × 10⁴	0.1	5	1.8 ± 0.37	3.8 ± 0.37	< .01	2.0
228	4 × 10⁴	0.1	5	0.0	2.0 ± 0.3	< .001	2.0

Absence of added PEG-rHu MGDF but in the presence of ATRA and IL-3.

Mean difference, 3.98; ^SE mean difference, 1.23;t = 3.23; n = 16; .01 >P > .001 (paired t test).

Student t test.