Immunocytologic analysis of human cells contained in the bone marrow of transplanted NOD/SCID mice
| Antihuman antibodies . | PBC-CD34+ . | CBC-MNC . | CBC-CD34+ . | |||
|---|---|---|---|---|---|---|
| No. of positive mice/total . | % positive* . | No. of positive mice/total . | % positive* . | No. of positive mice/total . | % positive* . | |
| CD34 | 3/10 | 1.2 ± 1.0 | 7/10 | 2.0 ± 0.8 | 8/8 | 6.9 ± 6.1 |
| CD33 | 7/10 | 2.1 ± 2.0 | 6/8 | 4.2 ± 2.1 | 14/8 | 3.3 ± 1.5 |
| CD38 | 5/10 | 8.1 ± 2.4 | 4/5 | 22.2 ± 6.8 | 8/8 | 37.1 ± 12.1 |
| HLA-I | 12/14 | 22.0 ± 10.4 | 5/5 | 22.0 ± 3.0 | 8/8 | 63.8 ± 22.8 |
| CD14/CD68 | 4/7 | 3.7 ± 2.2 | 4/5 | 3.8 ± 2.2 | 6/8 | 0.4 ± 0.2 |
| AS02* | 3/4 | 0.8 ± 0.3 | 5/6 | 3.6 ± 3.0 | 3/4 | 2.0 ± 0.8 |
| 5B5* | 3/4 | 0.8 ± 0.4 | ND | 5/6 | 2.4 ± 1.1 | |
| vWF | 2/5 | 0.6 ± 0.6 | 2/5 | 0.4 ± 0.3 | 8/8 | 0.6 ± 0.3 |
| KDR | ND | ND | 8/8 | 0.9 ± 0.2 | ||
| Antihuman antibodies . | PBC-CD34+ . | CBC-MNC . | CBC-CD34+ . | |||
|---|---|---|---|---|---|---|
| No. of positive mice/total . | % positive* . | No. of positive mice/total . | % positive* . | No. of positive mice/total . | % positive* . | |
| CD34 | 3/10 | 1.2 ± 1.0 | 7/10 | 2.0 ± 0.8 | 8/8 | 6.9 ± 6.1 |
| CD33 | 7/10 | 2.1 ± 2.0 | 6/8 | 4.2 ± 2.1 | 14/8 | 3.3 ± 1.5 |
| CD38 | 5/10 | 8.1 ± 2.4 | 4/5 | 22.2 ± 6.8 | 8/8 | 37.1 ± 12.1 |
| HLA-I | 12/14 | 22.0 ± 10.4 | 5/5 | 22.0 ± 3.0 | 8/8 | 63.8 ± 22.8 |
| CD14/CD68 | 4/7 | 3.7 ± 2.2 | 4/5 | 3.8 ± 2.2 | 6/8 | 0.4 ± 0.2 |
| AS02* | 3/4 | 0.8 ± 0.3 | 5/6 | 3.6 ± 3.0 | 3/4 | 2.0 ± 0.8 |
| 5B5* | 3/4 | 0.8 ± 0.4 | ND | 5/6 | 2.4 ± 1.1 | |
| vWF | 2/5 | 0.6 ± 0.6 | 2/5 | 0.4 ± 0.3 | 8/8 | 0.6 ± 0.3 |
| KDR | ND | ND | 8/8 | 0.9 ± 0.2 | ||
Bone marrow cells were fixed on glass slides, incubated with human-specific monoclonal antibodies and stained with immunoperoxidase as described in “Patients, materials, and methods.”
vWF indicates von Willebrand factor; ND, not determined; MNC, mononuclear cells (light-density fraction).
Clones of monoclonal antibodies recognizing epitopes specifically expressed on human fibroblasts (see “Patients, materials, and methods). Values are means ± SD of the positive (engrafted) mice.