Table 4. Comparison of the numbers and... | American Society of Hematology

Table 4.

Comparison of the numbers and frequency of cells expressing fibroblastic and endothelial markers before and after transplantation into NOD/SCID mice

Marker Graft type	Pretransplant (%)	Pretransplant (cells × 10⁵/mouse)	Posttransplant (cells × 10⁵/mouse)	Amplification factor
h-HLA-I
CBC-CD34⁺	100	1.5	732	488
CBC-MNC	100	110	727	6.6
PBC-CD34⁺	100	40	384	9.6
h-fibroblast (AS02 and/or 5B5)
CBC-CD34⁺	0.4	0.006	23.5	3917
CBC-MNC	0.9	0.99	47	52.2
PBC-CD34⁺	3.6	2.15	53	24.6
h-CD31
CBC-CD34⁺	0.1	0.083	94	1133
CBC-MNC	1.6	1.6	59	36.9
PBC-CD34⁺	2.0	0.74	47	63.5
h-vWF
CBC-CD34⁺	0.55	0.008	4.4	536
CBC-MNC	ND	ND	ND	ND
PBC-CD34⁺	0.3^4-150	0.07^4-150	2.3^4-150	31^4-150
h-flk-1/KDR
CBC-CD34⁺	ND	ND	132	>660^4-151
CBC-MNC	0.33^‡	0.4^‡	24^‡	60^‡
PBC-CD34⁺	ND	ND	ND	ND

Marker Graft type	Pretransplant (%)	Pretransplant (cells × 10⁵/mouse)	Posttransplant (cells × 10⁵/mouse)	Amplification factor
h-HLA-I
CBC-CD34⁺	100	1.5	732	488
CBC-MNC	100	110	727	6.6
PBC-CD34⁺	100	40	384	9.6
h-fibroblast (AS02 and/or 5B5)
CBC-CD34⁺	0.4	0.006	23.5	3917
CBC-MNC	0.9	0.99	47	52.2
PBC-CD34⁺	3.6	2.15	53	24.6
h-CD31
CBC-CD34⁺	0.1	0.083	94	1133
CBC-MNC	1.6	1.6	59	36.9
PBC-CD34⁺	2.0	0.74	47	63.5
h-vWF
CBC-CD34⁺	0.55	0.008	4.4	536
CBC-MNC	ND	ND	ND	ND
PBC-CD34⁺	0.3^4-150	0.07^4-150	2.3^4-150	31^4-150
h-flk-1/KDR
CBC-CD34⁺	ND	ND	132	>660^4-151
CBC-MNC	0.33^‡	0.4^‡	24^‡	60^‡
PBC-CD34⁺	ND	ND	ND	ND

Calculated are median values from 3 to 5 individual donors. Immunostaining was performed from cell suspensions as described in “Patients, materials, and methods.”

h indicates human; MNC, mononuclear cells (light-density fraction).

F4-150

Data from only two grafts.

F4-151

Calculated using pretransplant values from the literature.45

‡

Data from only one graft.

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