Determination of the proportion of intravenously injected human CRU that seed to the marrow of NOD/SCID mice within 24 hours
| Cells injected into mice . | Proportion of 1° BM injected per 2° mouse . | Proportion of negative mice . | No. CRU recovered per 1° mouse . | No. CRU injected per 1° mouse . | Seeding efficiency (%) . |
|---|---|---|---|---|---|
| CB | 0.675 | 1 of 7 | 3.4 | 48 | 7 |
| 0.075 | 4 of 6 | (2.2-5.2)† | (40-60) | ||
| FL | 0.675 | 0 of 2 | 2.8 | 54 | 5 |
| 0.45 | 2 of 9 | (2.0-3.9) | (45-64) | ||
| 0.075 | 2 of 2 | ||||
| 0.05 | 9 of 9 |
| Cells injected into mice . | Proportion of 1° BM injected per 2° mouse . | Proportion of negative mice . | No. CRU recovered per 1° mouse . | No. CRU injected per 1° mouse . | Seeding efficiency (%) . |
|---|---|---|---|---|---|
| CB | 0.675 | 1 of 7 | 3.4 | 48 | 7 |
| 0.075 | 4 of 6 | (2.2-5.2)† | (40-60) | ||
| FL | 0.675 | 0 of 2 | 2.8 | 54 | 5 |
| 0.45 | 2 of 9 | (2.0-3.9) | (45-64) | ||
| 0.075 | 2 of 2 | ||||
| 0.05 | 9 of 9 |
CRU indicates competitive repopulating unit; BM, bone marrow; CB, cord blood; FL, fetal liver.
Primary (1°) mice were injected with 5 × 107 CB cells or 2 × 107 FL cells and their marrow then removed 24 hours later for assessment of the number of human CRU present by transplantation of the harvested cells into secondary (2°) mice. Total recoveries of human CRU per 1° mouse were calculated based on the assumption that 2 femurs and 2 tibias contain 25% of the total marrow content of a mouse.12
Seeding efficiency values were calculated by dividing the total number of CRU injected per 1° mouse by the total number of CRU recovered per 1° mouse 24 hours later and multiplying by 100%.
Values shown in brackets are the range defined by the mean ± SEM and have been pooled from data obtained in the same 8 experiments with CB and FL cells described in Table 1.