Hemodynamic variation in observed vessels
| Stimulus . | Surgery . | Surgery . | Surgery . | Surgery . | TNFα . | TNFα . | TNFα . | TNFα . | TNFα . | TNFα . | TNFα . |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Bead coating | Control | PSGL-1 (293/FTIII) | PSGL-1 (293/FTIII) | PSGL-1 (293/FTIII) | Control | PSGL-1 (293/FTIII) | PSGL-1 (293/FTIII) | PSGL-1 (293/FTIII) | PSGL-1 (293/FTIII) | PSGL-1 (293/FTIII) | PSGL-1 (293/FTIII) |
| Mouse genotype | WT | WT | WT | WT | WT | WT | WT | WT | P−/− | E−/− | WT |
| Antibody | None | None | RB40.34 | RB6-8C5 | None | None | 10E6 | 10E6 + RB40.34 | None | None | RB6-8C5 |
| Vessel ∅ (μm) | 32 ± 3 | 33 ± 2 | 35 ± 3 | 35 ± 4 | 44 ± 3 | 40 ± 3 | 39 ± 2 | 39 ± 2 | 34 ± 3 | 36 ± 4 | 44 ± 3 |
| VC1 (mm/s) | 3.2 ± 0.3 | 3.0 ± 0.3 | 2.7 ± 0.4 | 2.1 ± 0.3 | 2.7 ± 0.3 | 2.8 ± 0.4 | 2.8 ± 0.5 | 2.4 ± 0.3 | 2.2 ± 0.3 | 3.2 ± 0.2 | 2.7 ± 0.3 |
| Stimulus . | Surgery . | Surgery . | Surgery . | Surgery . | TNFα . | TNFα . | TNFα . | TNFα . | TNFα . | TNFα . | TNFα . |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Bead coating | Control | PSGL-1 (293/FTIII) | PSGL-1 (293/FTIII) | PSGL-1 (293/FTIII) | Control | PSGL-1 (293/FTIII) | PSGL-1 (293/FTIII) | PSGL-1 (293/FTIII) | PSGL-1 (293/FTIII) | PSGL-1 (293/FTIII) | PSGL-1 (293/FTIII) |
| Mouse genotype | WT | WT | WT | WT | WT | WT | WT | WT | P−/− | E−/− | WT |
| Antibody | None | None | RB40.34 | RB6-8C5 | None | None | 10E6 | 10E6 + RB40.34 | None | None | RB6-8C5 |
| Vessel ∅ (μm) | 32 ± 3 | 33 ± 2 | 35 ± 3 | 35 ± 4 | 44 ± 3 | 40 ± 3 | 39 ± 2 | 39 ± 2 | 34 ± 3 | 36 ± 4 | 44 ± 3 |
| VC1 (mm/s) | 3.2 ± 0.3 | 3.0 ± 0.3 | 2.7 ± 0.4 | 2.1 ± 0.3 | 2.7 ± 0.3 | 2.8 ± 0.4 | 2.8 ± 0.5 | 2.4 ± 0.3 | 2.2 ± 0.3 | 3.2 ± 0.2 | 2.7 ± 0.3 |
Vessel diameter (∅) and centerline velocity (VC1) were measured for each microsphere coating, mouse genotype (WT = wild-type, E−/− = E-selectin knockout, P−/− = P-selectin knockout) and mAb (RB40.34 = anti–P-selectin, 10E6 = anti–E-selectin, RB6-8C5 = antineutrophil) treatment in surgically stimulated and TNFα-stimulated mice. Data are presented as mean ± SE.