IFN-α and IL-2 protect the clonal Th2 cells against spontaneous but not Fas-mediated apoptosis
| Agent added . | Spontaneous . | Fas-mediated . | ||
|---|---|---|---|---|
| Patient 1 . | Patient 2 . | Patient 1 . | Patient 2 . | |
| None | 28 ± 1 | 31 ± 3 | 89 ± 4 | 96 ± 2 |
| Anti-Fas mAb | 29 ± 2 | 28 ± 1 | 30 ± 3 | 28 ± 1 |
| Control mAb | 28 ± 1 | 28 ± 1 | 90 ± 4 | 97 ± 1 |
| IL-2 (100 IU/mL) | 11 ± 0.5* | 10 ± 1* | 78 ± 7 | 94 ± 1 |
| IFN-α (104IU/mL) | 9 ± 1* | 8 ± 0.5* | 83 ± 5 | 89 ± 4 |
| Agent added . | Spontaneous . | Fas-mediated . | ||
|---|---|---|---|---|
| Patient 1 . | Patient 2 . | Patient 1 . | Patient 2 . | |
| None | 28 ± 1 | 31 ± 3 | 89 ± 4 | 96 ± 2 |
| Anti-Fas mAb | 29 ± 2 | 28 ± 1 | 30 ± 3 | 28 ± 1 |
| Control mAb | 28 ± 1 | 28 ± 1 | 90 ± 4 | 97 ± 1 |
| IL-2 (100 IU/mL) | 11 ± 0.5* | 10 ± 1* | 78 ± 7 | 94 ± 1 |
| IFN-α (104IU/mL) | 9 ± 1* | 8 ± 0.5* | 83 ± 5 | 89 ± 4 |
Purified CD3−CD4+ cells from patient 1 and patient 2 were cultured for 24 hours in absence (spontaneous) or presence (Fas-mediated) of sFasL (25 ng/mL). Anti-Fas (1 μg/mL) or control mAb (1 μg/mL), IL-2 (100 IU/mL) or IFN-α (104IU/mL) was added at the beginning of the culture. At the end of the culture, apoptotic lymphocytes were detected by flow cytometry according to their light scatter properties (FSC/SSC). Results are the mean percentages ± SD of 3 experiments.
P < .05 as compared with culture without IFN-α or IL-2 (ANOVA test).