Expression of bcl-2 and bcl-xL in clonal Th2 cells
| Cells . | Agent added . | Bcl-xL . | Bcl-2 . | ||
|---|---|---|---|---|---|
| 0 . | 24 h . | 0 . | 24 h . | ||
| CD4+CD45RO+(controls) | — | 83 021 ± 6427 | 71 524 ± 4752 | 64 986 ± 5483 | 58 933 ± 4982 |
| CD3−CD4+ (patient 2) | — | 85 432 ± 5561 | — | 26 048 ± 6843* | — |
| CD3−CD4+ (patient 1) | — | 96 953 ± 11704 | 58 644 ± 10128 | 29 839 ± 5290* | 19 973 ± 2213 |
| +IFN-α (104IU/mL) | — | 62 563 ± 13723 | — | 19 911 ± 2555 | |
| +IL-2 (100 IU/mL) | — | 66 238 ± 22834 | — | 23 647 ± 3371† | |
| Cells . | Agent added . | Bcl-xL . | Bcl-2 . | ||
|---|---|---|---|---|---|
| 0 . | 24 h . | 0 . | 24 h . | ||
| CD4+CD45RO+(controls) | — | 83 021 ± 6427 | 71 524 ± 4752 | 64 986 ± 5483 | 58 933 ± 4982 |
| CD3−CD4+ (patient 2) | — | 85 432 ± 5561 | — | 26 048 ± 6843* | — |
| CD3−CD4+ (patient 1) | — | 96 953 ± 11704 | 58 644 ± 10128 | 29 839 ± 5290* | 19 973 ± 2213 |
| +IFN-α (104IU/mL) | — | 62 563 ± 13723 | — | 19 911 ± 2555 | |
| +IL-2 (100 IU/mL) | — | 66 238 ± 22834 | — | 23 647 ± 3371† | |
Intracytoplasmic bcl-xL and bcl-2 staining was performed on purified CD3+CD4+CD45RO+ cells from healthy individuals (n = 6) and CD3−CD4+ from hypereosinophilic patients (for each patient 6 determinations were performed). For patient 1, bcl-xL and bcl-2 levels were compared with those obtained after 24 hours of incubation in the absence or presence of IFN-α (104 IU/mL) or IL-2 (100 IU/mL). Results shown are the mean ± SD of bcl-xL and bcl-2 staining within live FSChigh/SSClow cells expressed in MESF.
P < .05 as compared with CD4+CD45RO+ cells from healthy individuals.
P < .05 as compared with bcl-2 expression obtained in absence of IL-2 (ANOVA test).