Properties of NPM, ALK, and NPM-ALK
| Property . | NPM . | ALK . | NPM-ALK . |
|---|---|---|---|
| Synonyms | B23, numatrin, NPM, NO38, nucleophosmin76 78 | ALK (anaplastic lymphoma kinase)76 | — |
| Chromosomal localization | 5q3576 | 2p2376 | 5q35 after t(2;5)76 |
| Molecular weight; length of cDNA (bp); number of amino acid residues (aa) | 32 500; about 1200 bp; 294 aa81 | 200 000; 6226 bp; 1620 aa83 84 | 80 000; 2040 bp; 680 aa (residues 1-116 from NPM; residue 117 generated by breakpoint; residues 118-680 from ALK corresponding to residue 1058-1620 of the ALK sequence)76 85 |
| Length of the gene; number of exons | 25 kB; 11 exons77 | No data available | Promoter and the first 4 exons of the NPM gene and the whole cytoplasmic domain of the ALK sequence77 |
| Cellular localization of the protein | Nucleolar81 | Transmembranous76,83 84 | Cytoplasmic and/or nuclear66,69 107 |
| Related proteins | Nucleoplasmin (nucleosome assembly factor)81 | Member of the insulin receptor family; LTK (leukocyte tyrosine kinase)76,83-85 103 | — |
| Function of the protein | Involved in preribosomal assembly, shuttles between nucleus and cytoplasm, represents one of the main silver-stained proteins (AgNORs)81 87 | Receptor tyrosine kinase with an unknown ligand; putative role during the development of the brain and peripheral neurons83 84 | Contains the oligomerization motif of NPM and the tyrosine kinase domain of ALK; malignant transformation in vitro86,91 107 and in vivo90 |
| Molecular occurrence | Homohexamer81 | Dimerization after stimulation/ligand binding83 84 | Homodimers and heterodimers86 107 |
| Expression pattern | Ubiquitous81 | Brain, particularly prenatal66,83 84 | All cells with t(2;5); expression regulated by the NPM promoter66,76 104 |
| Property . | NPM . | ALK . | NPM-ALK . |
|---|---|---|---|
| Synonyms | B23, numatrin, NPM, NO38, nucleophosmin76 78 | ALK (anaplastic lymphoma kinase)76 | — |
| Chromosomal localization | 5q3576 | 2p2376 | 5q35 after t(2;5)76 |
| Molecular weight; length of cDNA (bp); number of amino acid residues (aa) | 32 500; about 1200 bp; 294 aa81 | 200 000; 6226 bp; 1620 aa83 84 | 80 000; 2040 bp; 680 aa (residues 1-116 from NPM; residue 117 generated by breakpoint; residues 118-680 from ALK corresponding to residue 1058-1620 of the ALK sequence)76 85 |
| Length of the gene; number of exons | 25 kB; 11 exons77 | No data available | Promoter and the first 4 exons of the NPM gene and the whole cytoplasmic domain of the ALK sequence77 |
| Cellular localization of the protein | Nucleolar81 | Transmembranous76,83 84 | Cytoplasmic and/or nuclear66,69 107 |
| Related proteins | Nucleoplasmin (nucleosome assembly factor)81 | Member of the insulin receptor family; LTK (leukocyte tyrosine kinase)76,83-85 103 | — |
| Function of the protein | Involved in preribosomal assembly, shuttles between nucleus and cytoplasm, represents one of the main silver-stained proteins (AgNORs)81 87 | Receptor tyrosine kinase with an unknown ligand; putative role during the development of the brain and peripheral neurons83 84 | Contains the oligomerization motif of NPM and the tyrosine kinase domain of ALK; malignant transformation in vitro86,91 107 and in vivo90 |
| Molecular occurrence | Homohexamer81 | Dimerization after stimulation/ligand binding83 84 | Homodimers and heterodimers86 107 |
| Expression pattern | Ubiquitous81 | Brain, particularly prenatal66,83 84 | All cells with t(2;5); expression regulated by the NPM promoter66,76 104 |