Recovery of 32D cells from spleen of mice
| Experimental group . | n . | Cellularity × 10−7 . | G418R cfu/5 × 105cells . | G418R cfu/spleen . | P value vs ctl . | P value vs KitWT . |
|---|---|---|---|---|---|---|
| PBS | 10 | 6.7 | 0.0034 | 0.063 | n/a | .001 |
| 32D neo | 6 | 4.5 | 0.017 | 1.4 | n/a | .001 |
| 32D-KitWT | 16 | 11 | 200 | 41 299 | .001 | n/a |
| 32D-KitYF719 | 6 | 9.5 | 7.3 | 1 534 | .034 | .16 |
| 32D-KitYF728(cl1) | 5 | 6.8 | 4.2 | 81.7 | .262 | .03 |
| 32D-KitYF728(cl2) | 11 | 8.8 | 0.026 | 1.1 | .962 | .004 |
| 32D-KitYF728(sorted) | 5 | 9.5 | 0.1 | 18.2 | .551 | .001 |
| Experimental group . | n . | Cellularity × 10−7 . | G418R cfu/5 × 105cells . | G418R cfu/spleen . | P value vs ctl . | P value vs KitWT . |
|---|---|---|---|---|---|---|
| PBS | 10 | 6.7 | 0.0034 | 0.063 | n/a | .001 |
| 32D neo | 6 | 4.5 | 0.017 | 1.4 | n/a | .001 |
| 32D-KitWT | 16 | 11 | 200 | 41 299 | .001 | n/a |
| 32D-KitYF719 | 6 | 9.5 | 7.3 | 1 534 | .034 | .16 |
| 32D-KitYF728(cl1) | 5 | 6.8 | 4.2 | 81.7 | .262 | .03 |
| 32D-KitYF728(cl2) | 11 | 8.8 | 0.026 | 1.1 | .962 | .004 |
| 32D-KitYF728(sorted) | 5 | 9.5 | 0.1 | 18.2 | .551 | .001 |
Single cell suspensions from spleen of inoculated mice were assayed for 32D cell content 5 to 7 weeks after inoculation. The PBS control refers to mice injected with phosphate-buffered saline only. Cellularity refers to the arithmetic average of cell number per spleen samples. G418R colony-forming units (cfu) were enumerated 10 days after plating in agar. The reported numbers are the geometric means of each group. P values were determined using the unpaired, 2-tailed Student t test.