Recovery of 32D cells from bone marrow of mice
| Experimental group . | n . | Cellularity × 10−6 . | G418R cfu/5 × 105cells . | G418R cfu/femur . | P value vs ctl . | P value vs KitWT . |
|---|---|---|---|---|---|---|
| PBS | 10 | 4.1 | 0.00093 | 0.0031 | n/a | .001 |
| 32D neo | 6 | 1.7 | 0.00081 | 0.0024 | n/a | .001 |
| 32D-KitWT | 16 | 2.9 | 180 | 877 | .001 | n/a |
| 32D-KitYF719 | 6 | 4.1 | 58.2 | 373 | .002 | .347 |
| 32D-KitYF728(cl1) | 5 | 3.4 | 0.00083 | 0.0036 | .92 | .012 |
| 32D-KitYF728(cl2) | 11 | 2.8 | 0.049 | 0.2 | .329 | .004 |
| 32D-KitYF728(sorted) | 5 | 3.3 | 0.011 | 0.2 | .25 | .004 |
| Experimental group . | n . | Cellularity × 10−6 . | G418R cfu/5 × 105cells . | G418R cfu/femur . | P value vs ctl . | P value vs KitWT . |
|---|---|---|---|---|---|---|
| PBS | 10 | 4.1 | 0.00093 | 0.0031 | n/a | .001 |
| 32D neo | 6 | 1.7 | 0.00081 | 0.0024 | n/a | .001 |
| 32D-KitWT | 16 | 2.9 | 180 | 877 | .001 | n/a |
| 32D-KitYF719 | 6 | 4.1 | 58.2 | 373 | .002 | .347 |
| 32D-KitYF728(cl1) | 5 | 3.4 | 0.00083 | 0.0036 | .92 | .012 |
| 32D-KitYF728(cl2) | 11 | 2.8 | 0.049 | 0.2 | .329 | .004 |
| 32D-KitYF728(sorted) | 5 | 3.3 | 0.011 | 0.2 | .25 | .004 |
Single cell suspensions from bone marrow of inoculated mice were assayed for 32D cell content 5 to 7 weeks after inoculation. The PBS control refers to mice injected with phosphate-buffered saline only. Cellularity refers to the arithmetic average of cell number per femur in the bone marrow samples. G418R colony-forming units (cfu) were enumerated 10 days after planting in agar. The reported numbers are the geometric means of each group. P values were determined using the unpaired, 2-tailed Student ttest.