PTX3 does not influence the phagocytosis of inert substrates by immature dendritic cells
| Substrate . | Immature DCs . | TNF-α treated DCs . | ||
|---|---|---|---|---|
| − . | +PTX3 . | − . | +PTX3 . | |
| Apoptotic cells | 56 ± 8 | 21 ± 6 | 6 ± 5 | 8 ± 6 |
| Latex beads | 30 ± 9 | 28 ± 12 | 11 ± 5 | 11 ± 2 |
| Substrate . | Immature DCs . | TNF-α treated DCs . | ||
|---|---|---|---|---|
| − . | +PTX3 . | − . | +PTX3 . | |
| Apoptotic cells | 56 ± 8 | 21 ± 6 | 6 ± 5 | 8 ± 6 |
| Latex beads | 30 ± 9 | 28 ± 12 | 11 ± 5 | 11 ± 2 |
DC indicates dendritic cell; TNF-α, tumor necrosis factor-α.
The percentage of DCs, either immature or treated with TNF-α for 24 hours, that internalized particulate substrates (green fluorescent apoptotic cells or latex beads) in the absence or in the presence of PTX3 (50 μg/mL) was assessed by flow cytometry, as described in “Materials and methods.” Results represent the mean ± SEM of 3 separate experiments.