Table 1.

Number of colonies derived from osteoclast precursor cells

c-Fms+RANKc-Fms+RANK+c-Fms+RANK
No factor 0  
M-CSF alone 0.5 ± 0.5 0.8 ± 1.0 50.4 ± 10.4 
M-CSF + sRANKL 0.3 ± 0.5 0.3 ± 1.0 54.8 ± 10.5 
c-Fms+RANKc-Fms+RANK+c-Fms+RANK
No factor 0  
M-CSF alone 0.5 ± 0.5 0.8 ± 1.0 50.4 ± 10.4 
M-CSF + sRANKL 0.3 ± 0.5 0.3 ± 1.0 54.8 ± 10.5 

We cultured 3000 fractionated bone marrow mononuclear cells in methylcellulose medium with or without M-CSF or M-CSF and sRANKL for 6 days. The number of colonies (mean ± SD) was obtained from 3 separate experiments.

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