Depletion of NK cells using anti-asialo GM1 antibody in BALB/c.B6Cmv1rmice
| Tissue* . | n† . | No. leukocytes (×10−6)‡ . | % NK1.1+ NK cells . | % TCRβ+ cells . | % NK1.1+ TCRβ+cells . |
|---|---|---|---|---|---|
| Spleen | 5 | 105 ± 51-153 | 3.0 ± 0.4 | 25 ± 2 | 0.4 ± 0.1 |
| Spleen + Ab | 3 | 90 ± 7 | < 0.2 | 26 ± 3 | 0.5 ± 0.2 |
| Liver | 5 | 2.4 ± 0.4 | 19.7 ± 1.4 | 43 ± 4 | 7.7 ± 1.3 |
| Liver + Ab | 3 | 2.8 ± 0.1 | 0.3 ± 0.1 | 44 ± 4 | 7.5 ± 3.4 |
| Tissue* . | n† . | No. leukocytes (×10−6)‡ . | % NK1.1+ NK cells . | % TCRβ+ cells . | % NK1.1+ TCRβ+cells . |
|---|---|---|---|---|---|
| Spleen | 5 | 105 ± 51-153 | 3.0 ± 0.4 | 25 ± 2 | 0.4 ± 0.1 |
| Spleen + Ab | 3 | 90 ± 7 | < 0.2 | 26 ± 3 | 0.5 ± 0.2 |
| Liver | 5 | 2.4 ± 0.4 | 19.7 ± 1.4 | 43 ± 4 | 7.7 ± 1.3 |
| Liver + Ab | 3 | 2.8 ± 0.1 | 0.3 ± 0.1 | 44 ± 4 | 7.5 ± 3.4 |
Cell suspensions were prepared as described and labeled with mAb specific for αβTCR and NK1.1.
Mice were untreated or treated with 100 μg anti-asialo GM1 one day before tissue harvest.
n, number of mice tested.
Total number of leukocytes isolated from spleen or liver.21
Results represent mean ± SE.