Table 1.

Expression of BLyS on tumor cell lines

Cell lineCellular morphologyBLyS expression
(mean fluorescence values)
HL-60 acute promyelocytic leukemia 8.1  (3.3)  
THP-1 acute monocytic leukemia 17.1  (3.5)  
U937 histiocytic lymphoma 12.6  (4.5)  
Jurkat T-lymphocytic leukemia 2.9  (3.1)  
Sup-T1 T-lymphoblastic lymphoma 2.3  (1.8) 
Namalwa Burkitt's lymphoma 2.7  (2.2)  
Reh acute lymphocytic leukemia 3.8  (2.9)  
IM-9 multiple myeloma 3.8  (2.8) 
Cell lineCellular morphologyBLyS expression
(mean fluorescence values)
HL-60 acute promyelocytic leukemia 8.1  (3.3)  
THP-1 acute monocytic leukemia 17.1  (3.5)  
U937 histiocytic lymphoma 12.6  (4.5)  
Jurkat T-lymphocytic leukemia 2.9  (3.1)  
Sup-T1 T-lymphoblastic lymphoma 2.3  (1.8) 
Namalwa Burkitt's lymphoma 2.7  (2.2)  
Reh acute lymphocytic leukemia 3.8  (2.9)  
IM-9 multiple myeloma 3.8  (2.8) 

Levels of membrane-bound BLyS expression on myeloid cell lines were analyzed by flow cytometry. Cells were incubated with the mAb 2E5 or mouse IgG1 followed by PE-labeled goat antimouse F(ab′)2. Mean fluorescence values obtained with the isotype control are in parentheses.

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