Regulation of BLyS expression on monocytes by cytokine treatment
| Treatment . | BLyS expression (mean fluorescence values) . | |||
|---|---|---|---|---|
| Donor 1 . | Donor 2 . | Donor 3 . | Donor 4 . | |
| None | 4.2 (2.6) | 11.2 (5.3) | 2.0 (1.6) | 5.9 (2.7) |
| IFN-γ | 16.5 (2.4) | 62.0 (4.6) | 30.3 (1.5) | 30.6 (1.9) |
| IL-4 | 2.4 (2.2) | 3.6 (2.9) | 1.6 (1.1) | 2.1 (1.7) |
| IL-10 | 20.0 (3.6) | 43.6 (5.2) | 25.4 (1.6) | 58.8 (4.8) |
| IL-4 + IFN-γ | 3.7 (1.7) | 62.4 (3.1) | 72.8 (1.5) | 42.3 (1.6) |
| IL-4 + IL-10 | 3.6 (2.3) | 7.4 (2.6) | 2.0 (1.5) | 9.0 (2.0) |
| Treatment . | BLyS expression (mean fluorescence values) . | |||
|---|---|---|---|---|
| Donor 1 . | Donor 2 . | Donor 3 . | Donor 4 . | |
| None | 4.2 (2.6) | 11.2 (5.3) | 2.0 (1.6) | 5.9 (2.7) |
| IFN-γ | 16.5 (2.4) | 62.0 (4.6) | 30.3 (1.5) | 30.6 (1.9) |
| IL-4 | 2.4 (2.2) | 3.6 (2.9) | 1.6 (1.1) | 2.1 (1.7) |
| IL-10 | 20.0 (3.6) | 43.6 (5.2) | 25.4 (1.6) | 58.8 (4.8) |
| IL-4 + IFN-γ | 3.7 (1.7) | 62.4 (3.1) | 72.8 (1.5) | 42.3 (1.6) |
| IL-4 + IL-10 | 3.6 (2.3) | 7.4 (2.6) | 2.0 (1.5) | 9.0 (2.0) |
Following 3-day treatment with the cytokines, monocytes were incubated with anti-BLyS mAb 9B6 or mouse IgG1 and then with PE-labeled goat antimouse F(ab′)2. Cells were then analyzed using a FACScan. Mean fluorescence values obtained with the isotype control are in parentheses.