E-cadherin surface expression by cultured cells
| E-cad+ (MFI)* . | Experiment 1 . | Experiment 2 . |
|---|---|---|
| 1° culture day 10† | ||
| GM/SCF/FL/TNFα/TGF-β1 | 40.4 (31.3) | 41.5 (28.3) |
| 1° culture day 12† | ||
| GM/SCF/FL/TNFα/TGF-β1 | 41.0 (21.6) | 46.1 (20.7) |
| 2° culture‡ | ||
| GM-CSF | 32.9 (20.3) | 35.5 (23.8) |
| 2° culture | ||
| GM + TNFα | 31.3 (18.7) | 41.6 (21.8) |
| 2° culture | ||
| GM + CD40L | 33.1 (19.3) | 32.5 (21.2) |
| 2° culture | ||
| GM/SCF/FL/TNFα/TGF-β1 | 50.3 (24.7) | 49.3 (21.0) |
| E-cad+ (MFI)* . | Experiment 1 . | Experiment 2 . |
|---|---|---|
| 1° culture day 10† | ||
| GM/SCF/FL/TNFα/TGF-β1 | 40.4 (31.3) | 41.5 (28.3) |
| 1° culture day 12† | ||
| GM/SCF/FL/TNFα/TGF-β1 | 41.0 (21.6) | 46.1 (20.7) |
| 2° culture‡ | ||
| GM-CSF | 32.9 (20.3) | 35.5 (23.8) |
| 2° culture | ||
| GM + TNFα | 31.3 (18.7) | 41.6 (21.8) |
| 2° culture | ||
| GM + CD40L | 33.1 (19.3) | 32.5 (21.2) |
| 2° culture | ||
| GM/SCF/FL/TNFα/TGF-β1 | 50.3 (24.7) | 49.3 (21.0) |
E-cad indicates E-cadherin; MFI, mean fluorescence intensity; GM, granulocyte macrophage; SCF, stem cell factor; FL, flt3 ligand; TNFα, tumor necrosis factor-α; TGF-β1, transforming growth factor β1; CSF, colony-stimulating factor.
Mean fluorescence intensity of gated E-cadherin+ cells evaluated by fluorescence-activated cell sorter (FACS).
LCs generated in primary (1°) cultures of CD34+ cells in the presence of the indicated cytokines.
LCs harvested on day 10 and subcultured for 48 hours in secondary (2°) cultures in the presence of cytokines, as indicated.