KM1730-staining–positive (Flt-1+) macrophagelike cells differentiated from CD34+ cord blood cells
| Case no. . | CD11b+ . | CD97+ . | KM1730 . | |
|---|---|---|---|---|
| (+) . | (++) . | |||
| 1 | 57.8 | 98.1 | 52.1 | 26.0 |
| 2 | 54.9 | 96.4 | 56.9 | 19.3 |
| 3 | 66.8 | 99.4 | 23.3 | 18.9 |
| 4 | 33.0 | 99.5 | 46.1 | 15.7 |
| 5 | 50.5 | 98.8 | 55.3 | 25.6 |
| 6 | 49.7 | 97.8 | 34.7 | 15.1 |
| Mean | 52.1 | 98.3 | 44.7 | 20.1 |
| SD | 11.2 | 1.2 | 13.2 | 4.7 |
| Case no. . | CD11b+ . | CD97+ . | KM1730 . | |
|---|---|---|---|---|
| (+) . | (++) . | |||
| 1 | 57.8 | 98.1 | 52.1 | 26.0 |
| 2 | 54.9 | 96.4 | 56.9 | 19.3 |
| 3 | 66.8 | 99.4 | 23.3 | 18.9 |
| 4 | 33.0 | 99.5 | 46.1 | 15.7 |
| 5 | 50.5 | 98.8 | 55.3 | 25.6 |
| 6 | 49.7 | 97.8 | 34.7 | 15.1 |
| Mean | 52.1 | 98.3 | 44.7 | 20.1 |
| SD | 11.2 | 1.2 | 13.2 | 4.7 |
CD34+ but Flt-1− cells were obtained from human cord blood and differentiated into monocyte-macrophage lineages. Macrophagelike cell fraction was further analyzed with anti-CD11b, CD97, or Flt-1 (KM1730) antibody (see “Materials and methods”).