Table 1. Distribution of V gene... | American Society of Hematology

Table 1.

Distribution of V gene mutations in anti-HCV E2 hybridomas

Hybridomas	VH Germline	Homology (%)	R:S mutations		Scarcity of R mutation in FRs	Excess of R mutation in CDRs
Hybridomas	VH Germline	Homology (%)	FRs	CDRs	Scarcity of R mutation in FRs	Excess of R mutation in CDRs
CBH4B	VH1-69	90.8	8:8	9:1	P = .00300^*	P = .00925^*
CBH4D	VH1-69	93.3	8:3	3:5	P = .08676	P = .00011^*
CBH4G	VH1-69	92.9	5:5	7:3	P = .00176^*	P = .02163^*
CBH5	VH1-69	88.3	9:11	11:2	P = .00022^*	P = .00247^*
CBH7	VH1-69	90.8	9:10	6:1	P = .00925^*	P = .00019^*
CBH8E	VH1-69	96.4	3:3	5:2	P = .00691^*	P = .08563
CBH11	VH1-e	91.5	8:5	4:7	P = .00864^*	P = .00002^*
CBH2	VH5-51	94.3	6:4	6:0	P = .04465^*	P = .04465^*
CBH8C	VH4-59	92.8	1:7	9:3	P = .00001^*	P = .15227
CBH17	VH3-73	88.8	11:10	10:1	P = .00592^*	P = .00204^*

Hybridomas	VH Germline	Homology (%)	R:S mutations		Scarcity of R mutation in FRs	Excess of R mutation in CDRs
Hybridomas	VH Germline	Homology (%)	FRs	CDRs	Scarcity of R mutation in FRs	Excess of R mutation in CDRs
CBH4B	VH1-69	90.8	8:8	9:1	P = .00300^*	P = .00925^*
CBH4D	VH1-69	93.3	8:3	3:5	P = .08676	P = .00011^*
CBH4G	VH1-69	92.9	5:5	7:3	P = .00176^*	P = .02163^*
CBH5	VH1-69	88.3	9:11	11:2	P = .00022^*	P = .00247^*
CBH7	VH1-69	90.8	9:10	6:1	P = .00925^*	P = .00019^*
CBH8E	VH1-69	96.4	3:3	5:2	P = .00691^*	P = .08563
CBH11	VH1-e	91.5	8:5	4:7	P = .00864^*	P = .00002^*
CBH2	VH5-51	94.3	6:4	6:0	P = .04465^*	P = .04465^*
CBH8C	VH4-59	92.8	1:7	9:3	P = .00001^*	P = .15227
CBH17	VH3-73	88.8	11:10	10:1	P = .00592^*	P = .00204^*

Hybridomas	VL Germline	Homology (%)	R:S mutations		Scarcity of R mutation in FRs	Excess of R mutation in CDRs
Hybridomas	VL Germline	Homology (%)	FRs	CDRs	Scarcity of R mutation in FRs	Excess of R mutation in CDRs
CBH4B	Vk3-A27	95.4	3:3	4:2	P = .01085^*	P = .07773
CBH4D	Vλ2-2a2	95.6	6:3	3:0	P = .31691	P = .24510
CBH4G	Vk1-A20	98.5	0:0	4:0	P = .01350^*	P = .00026^*
CBH5	Vk1-L12	94.3	1:7	5:2	P = .00001^*	P = .05436
CBH7	Vk1-O12	96.2	6:2	1:1	P = .89335	P = .97264
CBH8E	Vk1-O12	97.3	0:4	3:0	P = .01453^*	P = .42831
CBH11	Vk1-L12	96.2	1:6	5:0	P = .00017^*	P = .02039^*
CBH2	Vk3-A27	96.2	1:2	5:2	P = .00110^*	P = .00848^*
CBH8C	Vk3-L6	98.1	1:1	3:0	P = .05049	P = .01699^*
CBH17	Vλ3-3h	90.9	10:4	8:2	P = .05545	P = .01976^*

Hybridomas	VL Germline	Homology (%)	R:S mutations		Scarcity of R mutation in FRs	Excess of R mutation in CDRs
Hybridomas	VL Germline	Homology (%)	FRs	CDRs	Scarcity of R mutation in FRs	Excess of R mutation in CDRs
CBH4B	Vk3-A27	95.4	3:3	4:2	P = .01085^*	P = .07773
CBH4D	Vλ2-2a2	95.6	6:3	3:0	P = .31691	P = .24510
CBH4G	Vk1-A20	98.5	0:0	4:0	P = .01350^*	P = .00026^*
CBH5	Vk1-L12	94.3	1:7	5:2	P = .00001^*	P = .05436
CBH7	Vk1-O12	96.2	6:2	1:1	P = .89335	P = .97264
CBH8E	Vk1-O12	97.3	0:4	3:0	P = .01453^*	P = .42831
CBH11	Vk1-L12	96.2	1:6	5:0	P = .00017^*	P = .02039^*
CBH2	Vk3-A27	96.2	1:2	5:2	P = .00110^*	P = .00848^*
CBH8C	Vk3-L6	98.1	1:1	3:0	P = .05049	P = .01699^*
CBH17	Vλ3-3h	90.9	10:4	8:2	P = .05545	P = .01976^*

The probability (P) that excess or scarcity of replacement (R) mutations in CDRs and in FRs resulted only by chance was calculated using the multinominal distribution model.24

Significant.

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