Effect of inhibitors of protein degradation and Brefeldin A on intracellular levels of VIIWT and VIIMT
| . | VIIWT, % . | VIIMT, % . |
|---|---|---|
| Media alone | 95 (±5) | 116 (±4) |
| NH4Cl (50 mM) | 121 (±4) | 115 (±3) |
| Leupeptin (100 μM) | 87 (±18) | 99 (±16) |
| Brefeldin A (10 μg/mL) | 150 (±3) | 88 (±24) |
| . | VIIWT, % . | VIIMT, % . |
|---|---|---|
| Media alone | 95 (±5) | 116 (±4) |
| NH4Cl (50 mM) | 121 (±4) | 115 (±3) |
| Leupeptin (100 μM) | 87 (±18) | 99 (±16) |
| Brefeldin A (10 μg/mL) | 150 (±3) | 88 (±24) |
Confluent CHO cells expressing recombinant VIIWT and VIIMT were incubated for 4 hours in fresh media containing 10% FBS in the presence or absence of agents. The results report the levels of VII:Ag in cell lysates, expressed as the percentage of VII:Ag (mean ± SE) from 3 independent experiments. The P values (see text) were calculated by the t test.