Sequences of the primers used for amplification of theb5R gene
| Exon and primer/fragment code . | Direction (sense or antisense) . | Primers . | Amplimer length (bp) . |
|---|---|---|---|
| FLAG | — | 5′ TGT AAA ACG ACG GCC AGT 3′ | — |
| Exon 1 | S | 5′ FLAG + CAG AAG CAG GCG GAG CGA CC 3′ | 252 |
| E1 | AS | 5′ GTC CCA GTC CCT CGC GAC GC 3′ | |
| Exon 2 | S | 5′ FLAG + TAG TGT GGA CAA GAA GCC GGT GT 3′ | 310 |
| E2 | AS | 5′ TAC TTC AGA GCA GAC CAT GC 3′ | |
| Exon 3 | AS | 5′ FLAG + ACG TCC CAG CTT CCT GCC TC 3′ | 352 |
| E3 | S | 5′ CGT TCA GCA GCT TTG GGC C 3′ | |
| Exon 4 | S | 5′ FLAG + GCA CCA GCA GGA GTG AAG GCA 3′ | 363 |
| E4 | AS | 5′ CAC CCA CAC CCC CTC CAC AG 3′ | |
| Exon 5 | S | 5′ FLAG + TGC AGA AGA GGC ACT TGT CC 3′ | 268 |
| E5 | AS | 5′ GTG TAA CCA AGG GAT TCC GA 3′ | |
| Exon 6 | S | 5′ FLAG + CCG GGC CTC ACC CCT TCT C 3′ | 473 |
| E6/7 | AS | 5′ CAG GGT AAG CTG AGT TTC CC 3′ | |
| Exon 7 | AS | 5′ FLAG + CAG GGT AAG CTG AGT TTC CC 3′ | 473 |
| E7/6 | S | 5′ CCG GGC CTC ACC CCT TCT C 3′ | |
| Exon 8 | S | 5′ FLAG + TGA AAG CTC CGC AAG CGT GT 3′ | 327 |
| E8 | AS | 5′ TCC AAA GCT CAA CGC CAC AA 3′ | |
| Exon 9 | S | 5′ FLAG + GCA TTT AAG AAT TCA CCG CC 3′ | 354 |
| E9 | AS | 5′ GGA GGT GAC TGG GTG AGC GT 3′ |
| Exon and primer/fragment code . | Direction (sense or antisense) . | Primers . | Amplimer length (bp) . |
|---|---|---|---|
| FLAG | — | 5′ TGT AAA ACG ACG GCC AGT 3′ | — |
| Exon 1 | S | 5′ FLAG + CAG AAG CAG GCG GAG CGA CC 3′ | 252 |
| E1 | AS | 5′ GTC CCA GTC CCT CGC GAC GC 3′ | |
| Exon 2 | S | 5′ FLAG + TAG TGT GGA CAA GAA GCC GGT GT 3′ | 310 |
| E2 | AS | 5′ TAC TTC AGA GCA GAC CAT GC 3′ | |
| Exon 3 | AS | 5′ FLAG + ACG TCC CAG CTT CCT GCC TC 3′ | 352 |
| E3 | S | 5′ CGT TCA GCA GCT TTG GGC C 3′ | |
| Exon 4 | S | 5′ FLAG + GCA CCA GCA GGA GTG AAG GCA 3′ | 363 |
| E4 | AS | 5′ CAC CCA CAC CCC CTC CAC AG 3′ | |
| Exon 5 | S | 5′ FLAG + TGC AGA AGA GGC ACT TGT CC 3′ | 268 |
| E5 | AS | 5′ GTG TAA CCA AGG GAT TCC GA 3′ | |
| Exon 6 | S | 5′ FLAG + CCG GGC CTC ACC CCT TCT C 3′ | 473 |
| E6/7 | AS | 5′ CAG GGT AAG CTG AGT TTC CC 3′ | |
| Exon 7 | AS | 5′ FLAG + CAG GGT AAG CTG AGT TTC CC 3′ | 473 |
| E7/6 | S | 5′ CCG GGC CTC ACC CCT TCT C 3′ | |
| Exon 8 | S | 5′ FLAG + TGA AAG CTC CGC AAG CGT GT 3′ | 327 |
| E8 | AS | 5′ TCC AAA GCT CAA CGC CAC AA 3′ | |
| Exon 9 | S | 5′ FLAG + GCA TTT AAG AAT TCA CCG CC 3′ | 354 |
| E9 | AS | 5′ GGA GGT GAC TGG GTG AGC GT 3′ |