Primers for polymerase chain reaction analysis ofIGK and IGL gene rearrangements
| Primers . | Sequence (5′-3′) . |
|---|---|
| IGKlocus | |
| LeaderVκI+II | GGTCCCCGCTCAGCTCCT |
| LeaderVκIV | TCTCTGTTGCTCTGGATCTCT |
| VκI | GTAGGAGACAGAGTCACCATCACT |
| VκII | TGGAGAGCCGGCCTCCATCTC |
| VκIII | GGGAAAGAGCCACCCTCTCCTG |
| VκIV | GGCGAGAGGGCCACCATCAAC |
| VκV | CCAGGAGACAAAGTCAACATCTCC |
| VκVI | CTGTGACTCCAAAGGAGAAAGTC |
| VκVII | AGGACAGAGGGCCACCATCACC |
| Jκ1,2,4 | CCCTGGTTCCACCTCTAGTTTGCA |
| Jκ3 | GGGACCAAAGTGGATATCAAACGT |
| Jκ5 | GGGACACGACTGGAGATTAAACGT |
| Cκ | ACTTTGGCCTCTCTGGGATA |
| IGLlocus | |
| LeaderVλI | GCCCAGTCTGTGCTGAC |
| LeaderVλII | CTGGGCTCTGCTCCTCCT |
| LeaderVλIII | GTGACCTCCTATGTGCTGACT |
| VλI | GGCAGAGGGTCACCATCTC |
| VλII | ATCTCCTGCACTGGAACCA |
| VλI+II | ATTCTCTGGCTCCAAGTCTGGCA |
| VλIII | ATTCTCTGGCTCCAACTCTGGGAA |
| Jλ1 | AGGCTGGGAAAGGTTGAG |
| Jλ2,3 | AGAGGGGAGAAGAGACTCAC |
| Cλ | TTGACGGGGCTGCTATCT |
| Primers . | Sequence (5′-3′) . |
|---|---|
| IGKlocus | |
| LeaderVκI+II | GGTCCCCGCTCAGCTCCT |
| LeaderVκIV | TCTCTGTTGCTCTGGATCTCT |
| VκI | GTAGGAGACAGAGTCACCATCACT |
| VκII | TGGAGAGCCGGCCTCCATCTC |
| VκIII | GGGAAAGAGCCACCCTCTCCTG |
| VκIV | GGCGAGAGGGCCACCATCAAC |
| VκV | CCAGGAGACAAAGTCAACATCTCC |
| VκVI | CTGTGACTCCAAAGGAGAAAGTC |
| VκVII | AGGACAGAGGGCCACCATCACC |
| Jκ1,2,4 | CCCTGGTTCCACCTCTAGTTTGCA |
| Jκ3 | GGGACCAAAGTGGATATCAAACGT |
| Jκ5 | GGGACACGACTGGAGATTAAACGT |
| Cκ | ACTTTGGCCTCTCTGGGATA |
| IGLlocus | |
| LeaderVλI | GCCCAGTCTGTGCTGAC |
| LeaderVλII | CTGGGCTCTGCTCCTCCT |
| LeaderVλIII | GTGACCTCCTATGTGCTGACT |
| VλI | GGCAGAGGGTCACCATCTC |
| VλII | ATCTCCTGCACTGGAACCA |
| VλI+II | ATTCTCTGGCTCCAAGTCTGGCA |
| VλIII | ATTCTCTGGCTCCAACTCTGGGAA |
| Jλ1 | AGGCTGGGAAAGGTTGAG |
| Jλ2,3 | AGAGGGGAGAAGAGACTCAC |
| Cλ | TTGACGGGGCTGCTATCT |