Table 3. Combined Southern blot and... | American Society of Hematology

Table 3.

Combined Southern blot and sequence data of the immunoglobulin κ⁺ chronic B-cell leukemia withIGL gene rearrangements and immunoglobulin λ⁺chronic B-cell leukemia with IGK gene rearrangements

Igκ⁺ CBL
Patient	IGK gene configuration^3-150	IGL gene configuration	IGLalleles							Somatic mutations^3-155
Patient	IGK gene configuration^3-150	IGL gene configuration	Vλ	Junctional region^3-151			Jλ	Frame^3-152	RT-PCR^3-153	IGK	IGL
1	R/VJ-Kde	R/G	Vλ3-21	0	GGGG	−3	Jλ1	+	ND	+	−
2	R/VJ-Kde	R/R	1st Vλ2-33	−3	AAA	−2	Jλ2	−^3-154	ND	+	−
			2nd not found
3	R/R#	R/G	Vλ1-40	0	−	0	Jλ3	+	+	NI	+

Igκ⁺ CBL
Patient	IGK gene configuration^3-150	IGL gene configuration	IGLalleles							Somatic mutations^3-155
Patient	IGK gene configuration^3-150	IGL gene configuration	Vλ	Junctional region^3-151			Jλ	Frame^3-152	RT-PCR^3-153	IGK	IGL
1	R/VJ-Kde	R/G	Vλ3-21	0	GGGG	−3	Jλ1	+	ND	+	−
2	R/VJ-Kde	R/R	1st Vλ2-33	−3	AAA	−2	Jλ2	−^3-154	ND	+	−
			2nd not found
3	R/R#	R/G	Vλ1-40	0	−	0	Jλ3	+	+	NI	+

Igλ⁺ CBL
	IGK gene configuration	IGL gene configuration	IGKalleles							Somatic mutations
	IGK gene configuration	IGL gene configuration	Vκ	Junctional region			Jκ	Frame	RT-PCR	IGK	IGL
4	R/VJ-Kde	R/R^3-160	1st not found							NI	+
			2nd Vκ1-39	−6	GA	−9	Jκ4	−	−
5	R/V-Kde	R/G	Vκ2-30	−10	TGACC	−4	Jκ2	−	ND	−	NI
6	R/V-Kde	R/G	Vκ2-28	−4	TCACT	−6	Jκ4	−	ND	−	−
7	R/V-Kde	R/G	Vκ1-33	−1	ACC	−6	Jκ4	−	ND	−	−
8	R/V-Kde	R/G	Vκ4-1	−3	CCTG	−2	Jκ2	+	+	−	−
9	R/VJ-Kde	R/G	1st Vκ1-33	−3	ATC	−2	Jκ3	+	+	−	−
			2nd Vκ4-1	−3	CT	0	Jκ4	−	−
10	R/VJ-Kde	R/G	1st Vκ1-12	−3	−	0	Jκ3	+	+	+	+
			2nd Vκ1-33	−3	AT	0	Jκ3	−	−

Igλ⁺ CBL
	IGK gene configuration	IGL gene configuration	IGKalleles							Somatic mutations
	IGK gene configuration	IGL gene configuration	Vκ	Junctional region			Jκ	Frame	RT-PCR	IGK	IGL
4	R/VJ-Kde	R/R^3-160	1st not found							NI	+
			2nd Vκ1-39	−6	GA	−9	Jκ4	−	−
5	R/V-Kde	R/G	Vκ2-30	−10	TGACC	−4	Jκ2	−	ND	−	NI
6	R/V-Kde	R/G	Vκ2-28	−4	TCACT	−6	Jκ4	−	ND	−	−
7	R/V-Kde	R/G	Vκ1-33	−1	ACC	−6	Jκ4	−	ND	−	−
8	R/V-Kde	R/G	Vκ4-1	−3	CCTG	−2	Jκ2	+	+	−	−
9	R/VJ-Kde	R/G	1st Vκ1-33	−3	ATC	−2	Jκ3	+	+	−	−
			2nd Vκ4-1	−3	CT	0	Jκ4	−	−
10	R/VJ-Kde	R/G	1st Vκ1-12	−3	−	0	Jκ3	+	+	+	+
			2nd Vκ1-33	−3	AT	0	Jκ3	−	−

Ig indicates immunoglobulin; RT-PCR, reverse transcriptase–polymerase chain reaction.

F3-150

R indicates IGK gene rearrangement; V-Kde, Vκ segment rearranged to kappa-deleting element; VJ-Kde, Vκ-Jκ and intron RSS-Kde rearrangement on the same allele.

F3-151

The junctional region is described as the number of bases lost at the 3′ end of the V segment followed by randomly inserted nucleotides and the number of bases lost from the 5′ end of the J segment.

F3-152

Frame of the rearrangement: +, in-frame, without stop codons in the junctional region; −, out-of-frame.

F3-153

ND indicates not determined; +, present; −, absent.

F3-155

+ indicates V segments with more than 2% somatic mutations; −, less than 2% somatic mutations; NI, not identified, because the rearrangement could not be amplified.

F3-154

A deletion of 36 nucleotides was found, starting from the last 2 codons of the Jλ2 gene segment up to 30 nucleotides in the J-Cλ intron, thereby deleting the Jλ splice site.

#For biallelic IGK gene rearrangements, see Table 4.

F3-160

For biallelic IGL gene rearrangements, see Table 5.

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