Table 5. Chronic B-cell leukemia with... | American Society of Hematology

Table 5.

Chronic B-cell leukemia with potentially functional biallelic Vλ-Jλ rearrangements as determined by Southern blotting

Patient	Vλ	Junctional region^5-150			Jλ	Frame^5-151	RT-PCR^5-152	Somatic mutations^5-153
4	Vλ1-44	0	G	−1	Jλ3	+	+	+
	Vλ2-5^5-155	−4	TGG	−1	Jλ3	−	+	+
20	Vλ1-40	−4	TCTA	0	Jλ3	+	+	+
	Vλ3-12	0	−	−3	Jλ3	+	+	+
21	Vλ3-21	0	−	0	Jλ3	+	ND	−
	Vλ1-47	0	A	0	Jλ3	−	ND	−
22	Vλ2-11	−15	26 nt of Vλ2-14	−7	Jλ3	+	+	+
	Vλ1-47	−4	C	0	Jλ3	+	+	+
23	Vλ1-40	−2	CGAG	−2	Jλ3	+	+	−
	Vλ3-21	−2	−	−2	Jλ3	−	+	−
24	Vλ3-21	0	CCCT	−1	Jλ3	+	+	−
	Vλ2-5^5-155	−4	−	−1	Jλ2	(+)^5-155	+	−
25	Vλ3-21	0	−	0	Jλ3	+	ND	−
	Vλ1-51	−11	CCC	0	Jλ3	−	ND	−
26	Vλ1-40	−5	AG	0	Jλ3	+	ND	+
	Vλ3-10	−6	GGGA	−4	Jλ1	−	ND	+
27	Vλ3-21	0	−		Jλ3	+	+	−
	NI				Jλ3^5-154		NI	NI
28	Vλ2-18	−3	GGT	0	Jλ1	+	+	+
	Vλ4-60	−2	−	−1	Jλ3	+	+	+
29	Vλ2-11	−3	CA	−3	Jλ3	+	+	+
	Vλ1-44	−2	−	0	Jλ3	+	+	−
30	Vλ2-14	−3	GG	−3	Jλ1	+	+	+
	NI				Jλ3^5-154		NI	NI
31	Vλ1-47	−6	−	−3	Jλ3	+	+	−
	NI				Jλ2^5-154		NI	NI
32	Vλ3-21	0	−	0	Jλ3	+	+	+
	Vλ1-47	−2	−	−1	Jλ3	+	+	+

Patient	Vλ	Junctional region^5-150			Jλ	Frame^5-151	RT-PCR^5-152	Somatic mutations^5-153
4	Vλ1-44	0	G	−1	Jλ3	+	+	+
	Vλ2-5^5-155	−4	TGG	−1	Jλ3	−	+	+
20	Vλ1-40	−4	TCTA	0	Jλ3	+	+	+
	Vλ3-12	0	−	−3	Jλ3	+	+	+
21	Vλ3-21	0	−	0	Jλ3	+	ND	−
	Vλ1-47	0	A	0	Jλ3	−	ND	−
22	Vλ2-11	−15	26 nt of Vλ2-14	−7	Jλ3	+	+	+
	Vλ1-47	−4	C	0	Jλ3	+	+	+
23	Vλ1-40	−2	CGAG	−2	Jλ3	+	+	−
	Vλ3-21	−2	−	−2	Jλ3	−	+	−
24	Vλ3-21	0	CCCT	−1	Jλ3	+	+	−
	Vλ2-5^5-155	−4	−	−1	Jλ2	(+)^5-155	+	−
25	Vλ3-21	0	−	0	Jλ3	+	ND	−
	Vλ1-51	−11	CCC	0	Jλ3	−	ND	−
26	Vλ1-40	−5	AG	0	Jλ3	+	ND	+
	Vλ3-10	−6	GGGA	−4	Jλ1	−	ND	+
27	Vλ3-21	0	−		Jλ3	+	+	−
	NI				Jλ3^5-154		NI	NI
28	Vλ2-18	−3	GGT	0	Jλ1	+	+	+
	Vλ4-60	−2	−	−1	Jλ3	+	+	+
29	Vλ2-11	−3	CA	−3	Jλ3	+	+	+
	Vλ1-44	−2	−	0	Jλ3	+	+	−
30	Vλ2-14	−3	GG	−3	Jλ1	+	+	+
	NI				Jλ3^5-154		NI	NI
31	Vλ1-47	−6	−	−3	Jλ3	+	+	−
	NI				Jλ2^5-154		NI	NI
32	Vλ3-21	0	−	0	Jλ3	+	+	+
	Vλ1-47	−2	−	−1	Jλ3	+	+	+

RT-PCR indicates reverse transcriptase–polymerase chain reaction.

F5-150

The junctional region is described as the number of bases lost at the 3′ end of the V segment followed by randomly inserted nucleotides and the number of bases lost from the 5′ end of the J segment.

F5-151

Frame of the junctional region: +, in-frame, without stop codons in the junctional region; −, out-of-frame.

F5-152

ND, not determined; NI, not identified; +, present; −, absent.

F5-153

+ indicates V segments with more than 2% somatic mutations; −, less than 2% somatic mutations; NI, not identified, because the rearrangement could not be amplified.

F5-155

Vλ2-5 is a pseudogene and, therefore, not functional.

F5-154

Based on Southern blot data.

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