Table 2.

Results indicating that early growth response gene 1 enhances macrophage differentiation in bone marrow cells of sublethally irradiated mice

Cells transplanted*Mice surviving (%)†No. of spleen colonies‡No. of femoral BM cells (×106)2-153Adherent cells (%)2-155Cell type (%)2-154
BlastGranulocytesMacrophagesOthers
BM normal 100 13 7.0 24 31 46 22 1  
BM mock 100 12 5.5 25 33 44 24 2 
BMneo 100 13 5.0 24 39 37 22 2 
BMEgr-1 100 6 3.0 50 23 25 51 0 
Cells transplanted*Mice surviving (%)†No. of spleen colonies‡No. of femoral BM cells (×106)2-153Adherent cells (%)2-155Cell type (%)2-154
BlastGranulocytesMacrophagesOthers
BM normal 100 13 7.0 24 31 46 22 1  
BM mock 100 12 5.5 25 33 44 24 2 
BMneo 100 13 5.0 24 39 37 22 2 
BMEgr-1 100 6 3.0 50 23 25 51 0 

For abbreviations, see Table 1.

*

Each BM type was transplanted into 13 sublethally irradiated mice (.2 × 106 cells/mouse).

†

For each BM type, 10 mice were monitored for survival for 6 weeks.

‡

Spleen colonies were counted on day 8. Values are mean (with SDs up to ±20%) results for spleens obtained from 3 mice.

F2-153

BM cells were collected from femurs 8 days after transplantation. Values are mean numbers of BM cells (with SDs up to ±15%) obtained from femurs of 3 mice.

F2-155

See Figure 8.

F2-154

Eight days after transplantation, BM cells were recovered from femurs and 0.3 × 106 cells/mL were seeded in liquid culture medium supplemented with interleukin 3 (10% WEHI-3B–conditioned medium). After 3 days, cytospin smears were prepared. At least 300 May-Grünwald–stained cells were scored. Values are mean (SD up to ±15%) results from 3 smears prepared from triplicate cultures.

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