Table 1. Sizes and primer sequences of... | American Society of Hematology

Table 1.

Sizes and primer sequences of PCR fragments for SSCP analysis of the promoter and the 3′UTR of the TAFI gene

Primer	Primer sequences	DNA position^* (5′ to 3′)	PCR size (bp)	Annealing temperature (°C)	Enzyme used before SSCP
Promoter
T1a	CCTCATAAAGCAGTGCAAC	− 2676	296	58
T1b	GTATGCTGTATTCTGGGTG	− 2380
T2a	CACCTCAACTGGACTATGT	− 2474	404	58	RsaI
T2b	TGTTCCCTTGCAGTTTAGC	− 2070
T3a	ATGGATCTGAGAAGCACC	− 2150	367	60	TaqI
T3b	TTTCCTCTCTGGGGAAAC	− 1783
T4a	CACCTGTAGACTTTTGC	− 1971	357	59
T4b	CACTGAAGGAGAGAAAG	− 1614
T5a	TTTTCTTTCTCTCCTTCAGTG	− 1634	260	59
T5b	ATCAATGAGACTGGTTATCCA	− 1374
T6a	ACTTCACCCAAAGAAGC	− 1467	282	59
T6b	CCTGGCCAGAAGAAAAT	− 1185
T7a	ATTTTCTTCTGGCCAGG	− 1200	435	61	Hinf1
T7b	CCTACATTCCCATTGGT	− 765
T8a	ACCAATGGGAATGTAGG	− 782	385	59	MboI
T8b	CCTTGCCTCTGACTTTT	− 397
T9a	TGGATTGGATTAGCCAG	− 476	322	59
T9b	TTGGCTTAGTTAGGCTG	− 154
T10a	CCCAAGGAGTTACACAT	− 254	279	59
T10b	AAGCTTCATCCCAACAG	− 25
3′ part of exon 10 + 3′UTR
3′a	CTTGGCATGTCATTAGG	+1268	612	59	BamHI
3′b	TGCGGCATTTGTTGACA	+ 1880

Primer	Primer sequences	DNA position^* (5′ to 3′)	PCR size (bp)	Annealing temperature (°C)	Enzyme used before SSCP
Promoter
T1a	CCTCATAAAGCAGTGCAAC	− 2676	296	58
T1b	GTATGCTGTATTCTGGGTG	− 2380
T2a	CACCTCAACTGGACTATGT	− 2474	404	58	RsaI
T2b	TGTTCCCTTGCAGTTTAGC	− 2070
T3a	ATGGATCTGAGAAGCACC	− 2150	367	60	TaqI
T3b	TTTCCTCTCTGGGGAAAC	− 1783
T4a	CACCTGTAGACTTTTGC	− 1971	357	59
T4b	CACTGAAGGAGAGAAAG	− 1614
T5a	TTTTCTTTCTCTCCTTCAGTG	− 1634	260	59
T5b	ATCAATGAGACTGGTTATCCA	− 1374
T6a	ACTTCACCCAAAGAAGC	− 1467	282	59
T6b	CCTGGCCAGAAGAAAAT	− 1185
T7a	ATTTTCTTCTGGCCAGG	− 1200	435	61	Hinf1
T7b	CCTACATTCCCATTGGT	− 765
T8a	ACCAATGGGAATGTAGG	− 782	385	59	MboI
T8b	CCTTGCCTCTGACTTTT	− 397
T9a	TGGATTGGATTAGCCAG	− 476	322	59
T9b	TTGGCTTAGTTAGGCTG	− 154
T10a	CCCAAGGAGTTACACAT	− 254	279	59
T10b	AAGCTTCATCCCAACAG	− 25
3′ part of exon 10 + 3′UTR
3′a	CTTGGCATGTCATTAGG	+1268	612	59	BamHI
3′b	TGCGGCATTTGTTGACA	+ 1880

DNA numbering of the TAFI promoter region is according to a proposed transcription start site (9). DNA numbering of the TAFI cDNA sequence is according to (13) and (9) for the end of the 3′UTR.

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