Effect of VEGF neutralization with rhu-mAb-VEGF on bone marrow progenitor growth
| UPN . | FAB type . | Immunohistochemistry . | Control (colony no. ± SD) . | rhu-mAb-VEGF (colony no. ± SD) . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| VEGF . | Flt-1 . | KDR . | CFU-GEMM . | BFU-E . | CFU-GM . | CFU-GEMM . | BFU-E . | CFU-GM . | ||
| UAZ001 | N1 Donor | 0 | 0 | 0 | 301 ± 16.2 | 692 ± 99 | 1257 ± 164 | 290 ± 13.6 | 718 ± 135 | 1361 ± 109 |
| UAZ002 | N1 Donor | 0 | 0 | 0 | 89 ± 8 | 570 ± 161 | 745 ± 57 | 107 ± 17.5 | 676 ± 44 | 839 ± 41 |
| UAZ003 | RA | 3 | 3 | 0 | 1.7 ± 1.5 | 2.7 ± 0.6 | 123 ± 33 | 7 ± 4.3 | 11.3 ± 44-150 | 190 ± 70 |
| UAZ004 | RA | 1 | 2 | 0 | 6.3 ± 4.2 | 47 ± 11 | 224 ± 37 | 19.3 ± 5.04-150 | 67 ± 23 | 222 ± 15 |
| UAZ005 | RA | ND | ND | ND | 1.3 ± 0.6 | 11 ± 8 | 47 ± 19 | 2.7 ± 1.5 | 14 ± 4.5 | 49 ± 17.6 |
| UAZ006 | RAEB | 0 | 0 | 0 | 1 ± 1 | 0.3 ± 0.6 | 723 ± 173 | 1 ± 0.6 | 1 ± 0 | 940 ± 450 |
| UAZ007 | RAEB | 3 | 1 | 0 | 13 ± 6.5 | 6.7 ± 3 | 1122 ± 244 | 15 ± 4.6 | 22 ± 9.14-150 | 1376 ± 369 |
| UAZ008 | RAEB | 2 | 2 | 0 | 0.3 ± 0.5 | 0.7 ± 1.1 | 93 | 1.6 ± 1.6 | 6 ± 5.3 | 441 |
| UAZ009 | RAEB | 2 | 2 | 1 | 6.7 ± 4.6 | 41 ± 11 | 1380 | 10 ± 2 | 77 ± 134-150 | 1345 |
| UAZ010 | RAEB | 3 | 1 | 0 | 8.3 ± 4.1 | 54 ± 13 | 730 ± 54 | 16.7 ± 2.34-150 | 82 ± 114-150 | 727 ± 36 |
| UAZ011 | RAEB-t | 1 | 1 | 0 | 0 | 1.7 ± 2.8 | 79 ± 14.7 | 0 | 1.7 ± 1.5 | 121 ± 12 |
| UAZ012 | RAEB-t | 4 | 3 | 3 | 18.7 ± 8 | 15.3 ± 26 | 193 ± 124 | 18.7 ± 3 | 39 ± 4 | 179 ± 46 |
| UAZ013 | RAEB-t | 4 | 3 | 3 | 70 ± 18.7 | 88 ± 39 | 1759 ± 300 | 105 ± 134-150 | 141 ± 304-150 | 2072 ± 375 |
| UAZ014 | RARS | 1 | 2 | 0 | 36.7 ± 6.4 | 53 ± 12 | 801 ± 136 | 62 ± 9.14-150 | 54 ± 20 | 791 ± 87 |
| UAZ015 | RARS | 4 | 2 | 0 | 84.7 ± 12 | 543 ± 83 | 1178 ± 250 | 175.3 ± 394-150 | 776 ± 614-150 | 1313 ± 113 |
| UPN . | FAB type . | Immunohistochemistry . | Control (colony no. ± SD) . | rhu-mAb-VEGF (colony no. ± SD) . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| VEGF . | Flt-1 . | KDR . | CFU-GEMM . | BFU-E . | CFU-GM . | CFU-GEMM . | BFU-E . | CFU-GM . | ||
| UAZ001 | N1 Donor | 0 | 0 | 0 | 301 ± 16.2 | 692 ± 99 | 1257 ± 164 | 290 ± 13.6 | 718 ± 135 | 1361 ± 109 |
| UAZ002 | N1 Donor | 0 | 0 | 0 | 89 ± 8 | 570 ± 161 | 745 ± 57 | 107 ± 17.5 | 676 ± 44 | 839 ± 41 |
| UAZ003 | RA | 3 | 3 | 0 | 1.7 ± 1.5 | 2.7 ± 0.6 | 123 ± 33 | 7 ± 4.3 | 11.3 ± 44-150 | 190 ± 70 |
| UAZ004 | RA | 1 | 2 | 0 | 6.3 ± 4.2 | 47 ± 11 | 224 ± 37 | 19.3 ± 5.04-150 | 67 ± 23 | 222 ± 15 |
| UAZ005 | RA | ND | ND | ND | 1.3 ± 0.6 | 11 ± 8 | 47 ± 19 | 2.7 ± 1.5 | 14 ± 4.5 | 49 ± 17.6 |
| UAZ006 | RAEB | 0 | 0 | 0 | 1 ± 1 | 0.3 ± 0.6 | 723 ± 173 | 1 ± 0.6 | 1 ± 0 | 940 ± 450 |
| UAZ007 | RAEB | 3 | 1 | 0 | 13 ± 6.5 | 6.7 ± 3 | 1122 ± 244 | 15 ± 4.6 | 22 ± 9.14-150 | 1376 ± 369 |
| UAZ008 | RAEB | 2 | 2 | 0 | 0.3 ± 0.5 | 0.7 ± 1.1 | 93 | 1.6 ± 1.6 | 6 ± 5.3 | 441 |
| UAZ009 | RAEB | 2 | 2 | 1 | 6.7 ± 4.6 | 41 ± 11 | 1380 | 10 ± 2 | 77 ± 134-150 | 1345 |
| UAZ010 | RAEB | 3 | 1 | 0 | 8.3 ± 4.1 | 54 ± 13 | 730 ± 54 | 16.7 ± 2.34-150 | 82 ± 114-150 | 727 ± 36 |
| UAZ011 | RAEB-t | 1 | 1 | 0 | 0 | 1.7 ± 2.8 | 79 ± 14.7 | 0 | 1.7 ± 1.5 | 121 ± 12 |
| UAZ012 | RAEB-t | 4 | 3 | 3 | 18.7 ± 8 | 15.3 ± 26 | 193 ± 124 | 18.7 ± 3 | 39 ± 4 | 179 ± 46 |
| UAZ013 | RAEB-t | 4 | 3 | 3 | 70 ± 18.7 | 88 ± 39 | 1759 ± 300 | 105 ± 134-150 | 141 ± 304-150 | 2072 ± 375 |
| UAZ014 | RARS | 1 | 2 | 0 | 36.7 ± 6.4 | 53 ± 12 | 801 ± 136 | 62 ± 9.14-150 | 54 ± 20 | 791 ± 87 |
| UAZ015 | RARS | 4 | 2 | 0 | 84.7 ± 12 | 543 ± 83 | 1178 ± 250 | 175.3 ± 394-150 | 776 ± 614-150 | 1313 ± 113 |
P < .05 versus controls.
ND, not done.