Colony formation from 8.5-dpc-YS and adult BM cells in the presence of various cytokines
| FBS . | Cytokines . | No. of colonies . | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 8.5 dpc YS . | Adult BM . | ||||||||||||
| E . | Mk . | EMk . | Mk-Mix . | Others . | Total . | E . | Mk . | EMk . | Mk-Mix . | Others . | Total . | ||
| + | None | 12 ± 2 | 28 ± 4 | 0 | 0 | 10 ± 2 | 50 ± 4 | 0 | 0 | 0 | 0 | 0 | 0 |
| TPO | 13 ± 4 | 72 ± 6* | 0 | 0 | 24 ± 4 | 109 ± 10 | 0 | 4 ± 1 | 0 | 2 ± 1 | 7 ± 2 | 13 ± 3 | |
| SCF | 25 ± 3 | 62 ± 4* | 0 | 0 | 39 ± 3 | 126 ± 13 | 0 | 0 | 0 | 0 | 74 ± 6 | 74 ± 6 | |
| IL-3 | 23 ± 3 | 92 ± 8* | 11 ± 2 | 20 ± 3 | 57 ± 4 | 203 ± 24 | 0 | 5 ± 1 | 0 | 4 ± 1 | 208 ± 14 | 217 ± 14 | |
| EPO | 21 ± 2 | 47 ± 6* | 9 ± 1 | 0 | 12 ± 3 | 89 ± 8 | 4 ± 1 | 0 | 0 | 0 | 0 | 0 | |
| − | None | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| TPO | 0 | 2 ± 1 | 0 | 0 | 0 | 2 ± 1 | 0 | 2 ± 1 | 0 | 0 | 0 | 2 ± 1 | |
| SCF | 3 ± 1 | 3 ± 1 | 0 | 0 | 9 ± 2 | 15 ± 2 | 0 | 0 | 0 | 0 | 18 ± 2 | 18 ± 2 | |
| IL-3 | 0 | 21 ± 3 | 0 | 6 ± 2 | 31 ± 3 | 58 ± 7 | 0 | 2 ± 1 | 0 | 1 ± 1 | 51 ± 5 | 54 ± 6 | |
| EPO | 9 ± 2 | 3 ± 1 | 2 ± 1 | 0 | 0 | 14 ± 2 | 0 | 0 | 0 | 0 | 0 | 0 | |
| FBS . | Cytokines . | No. of colonies . | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 8.5 dpc YS . | Adult BM . | ||||||||||||
| E . | Mk . | EMk . | Mk-Mix . | Others . | Total . | E . | Mk . | EMk . | Mk-Mix . | Others . | Total . | ||
| + | None | 12 ± 2 | 28 ± 4 | 0 | 0 | 10 ± 2 | 50 ± 4 | 0 | 0 | 0 | 0 | 0 | 0 |
| TPO | 13 ± 4 | 72 ± 6* | 0 | 0 | 24 ± 4 | 109 ± 10 | 0 | 4 ± 1 | 0 | 2 ± 1 | 7 ± 2 | 13 ± 3 | |
| SCF | 25 ± 3 | 62 ± 4* | 0 | 0 | 39 ± 3 | 126 ± 13 | 0 | 0 | 0 | 0 | 74 ± 6 | 74 ± 6 | |
| IL-3 | 23 ± 3 | 92 ± 8* | 11 ± 2 | 20 ± 3 | 57 ± 4 | 203 ± 24 | 0 | 5 ± 1 | 0 | 4 ± 1 | 208 ± 14 | 217 ± 14 | |
| EPO | 21 ± 2 | 47 ± 6* | 9 ± 1 | 0 | 12 ± 3 | 89 ± 8 | 4 ± 1 | 0 | 0 | 0 | 0 | 0 | |
| − | None | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| TPO | 0 | 2 ± 1 | 0 | 0 | 0 | 2 ± 1 | 0 | 2 ± 1 | 0 | 0 | 0 | 2 ± 1 | |
| SCF | 3 ± 1 | 3 ± 1 | 0 | 0 | 9 ± 2 | 15 ± 2 | 0 | 0 | 0 | 0 | 18 ± 2 | 18 ± 2 | |
| IL-3 | 0 | 21 ± 3 | 0 | 6 ± 2 | 31 ± 3 | 58 ± 7 | 0 | 2 ± 1 | 0 | 1 ± 1 | 51 ± 5 | 54 ± 6 | |
| EPO | 9 ± 2 | 3 ± 1 | 2 ± 1 | 0 | 0 | 14 ± 2 | 0 | 0 | 0 | 0 | 0 | 0 | |
A total of 5 × 104 day 8.5 YS and adult BM cells were plated using the clonal assay methods. The number of colonies indicates mean ± SD of triplicate cultures.
Significantly different from colony formation in the culture without cytokines (P < .001).