Binding of FITC-Rituxan (monomers and homodimers) to different cell lines
| Cells . | % Positive . | MFI . | Relative binding affinity (×10−10 M) . | |||
|---|---|---|---|---|---|---|
| Monomer . | Dimer . | Monomer . | Dimer . | Monomer . | Dimer . | |
| Daudi | 93.7 | 91.5 | 750 | 452 | 4.3 | 1.5 |
| DHL-4 | 99.8 | 99.6 | 467 | 545 | 0.5 | 1.5 |
| Ramos | 97.7 | 99.1 | 617 | 341 | 2.8 | 2.1 |
| Raji | 99.1 | 99.2 | 1358 | 670 | 1.7 | 2.4 |
| Namalwa | 54.0 | 47.4 | 93 | 121 | 400 | 430 |
| Namalwa/MDR1 | 65.3 | 39.3 | 108 | 99 | 670 | 730 |
| Jurkat | 5.3 | 4.5 | 16 | 37 | — | — |
| Cells . | % Positive . | MFI . | Relative binding affinity (×10−10 M) . | |||
|---|---|---|---|---|---|---|
| Monomer . | Dimer . | Monomer . | Dimer . | Monomer . | Dimer . | |
| Daudi | 93.7 | 91.5 | 750 | 452 | 4.3 | 1.5 |
| DHL-4 | 99.8 | 99.6 | 467 | 545 | 0.5 | 1.5 |
| Ramos | 97.7 | 99.1 | 617 | 341 | 2.8 | 2.1 |
| Raji | 99.1 | 99.2 | 1358 | 670 | 1.7 | 2.4 |
| Namalwa | 54.0 | 47.4 | 93 | 121 | 400 | 430 |
| Namalwa/MDR1 | 65.3 | 39.3 | 108 | 99 | 670 | 730 |
| Jurkat | 5.3 | 4.5 | 16 | 37 | — | — |
Data are based on 1 experiment of 2. Cells were stained with 10 μg/mL per 106 cells fluorescein isothiocyanate (FITC)-Rituxan (ie, saturating conditions) and analyzed by FACScan. Both FITC-Rituxan monomers and homodimers were titrated on different cell lines, and then the percentage of positive cells was plotted vs the monoclonal antibody concentration to determine the concentration necessary to reach 50% saturation of cells. This value represents the relative binding affinity.
MFI indicates mean fluorescence intensity.