Effect of chemokine pretreatment of monocytes and transfected cells expressing CCR2 or CCR5
| Cells . | Pretreatment . | Rate of [Ca++]i rise after stimulation . | |||
|---|---|---|---|---|---|
| Eotaxin . | MCP-1 . | RANTES . | MIP-1β . | ||
| Monocytes | |||||
| None | 100 | 100 | 100 | 100 | |
| Eotaxin | 57 | 64 | 33 | ||
| MCP-1 | 100 | 50 | |||
| RANTES | 0 | 50 | |||
| MIP-1β | 35 | 50 | |||
| CCR2-transfected cells | |||||
| None | 100 | ||||
| Eotaxin | 21 | ||||
| CCR5-transfected cells | |||||
| None | 100 | ||||
| Eotaxin | 22 | ||||
| Cells . | Pretreatment . | Rate of [Ca++]i rise after stimulation . | |||
|---|---|---|---|---|---|
| Eotaxin . | MCP-1 . | RANTES . | MIP-1β . | ||
| Monocytes | |||||
| None | 100 | 100 | 100 | 100 | |
| Eotaxin | 57 | 64 | 33 | ||
| MCP-1 | 100 | 50 | |||
| RANTES | 0 | 50 | |||
| MIP-1β | 35 | 50 | |||
| CCR2-transfected cells | |||||
| None | 100 | ||||
| Eotaxin | 21 | ||||
| CCR5-transfected cells | |||||
| None | 100 | ||||
| Eotaxin | 22 | ||||
The cells were stimulated sequentially with different chemokines, and changes of intracellular free calcium ([Ca++]i) were monitored as shown in Figures1 and 2. The rate of the [Ca++]i rises in the absence of stimulation was set to 100%, and the percentage rate obtained after prestimulation was calculated.