Regakine-1 has no chemokinetic activity on neutrophils
| Chemokine . | Concentration (ng/mL) . | Migration of neutrophils* . | |
|---|---|---|---|
| Simultaneous . | Preincubation . | ||
| Regakine-1 | 1000 | 0.4 ± 0.2 (7) | 0.9 ± 0.4 (3) |
| 300 | 1.1 ± 0.3 (9) | 1.4 ± 0.4 (11) | |
| 100 | 1.2 ± 0.3 (9) | 2.1 ± 0.5 (12) | |
| 30 | 0.5 ± 0.2 (6) | 1.3 ± 0.4 (5) | |
| MCP-3 | 300 | 0.3 ± 0.1 (3) | 1.6 ± 0.8 (3) |
| 100 | 1.8 ± 1.0 (3) | 2.0 ± 0.7 (3) | |
| 30 | 0.8 ± 0.5 (3) | 2.5 ± 1.0 (3) | |
| IL-8† | 50 | 0.2 ± 0.0 (2) | ND |
| 15 | 0.8 ± 0.3 (2) | ND | |
| Chemokine . | Concentration (ng/mL) . | Migration of neutrophils* . | |
|---|---|---|---|
| Simultaneous . | Preincubation . | ||
| Regakine-1 | 1000 | 0.4 ± 0.2 (7) | 0.9 ± 0.4 (3) |
| 300 | 1.1 ± 0.3 (9) | 1.4 ± 0.4 (11) | |
| 100 | 1.2 ± 0.3 (9) | 2.1 ± 0.5 (12) | |
| 30 | 0.5 ± 0.2 (6) | 1.3 ± 0.4 (5) | |
| MCP-3 | 300 | 0.3 ± 0.1 (3) | 1.6 ± 0.8 (3) |
| 100 | 1.8 ± 1.0 (3) | 2.0 ± 0.7 (3) | |
| 30 | 0.8 ± 0.5 (3) | 2.5 ± 1.0 (3) | |
| IL-8† | 50 | 0.2 ± 0.0 (2) | ND |
| 15 | 0.8 ± 0.3 (2) | ND | |
ND indicates not determined; MCP-3, monocyte chemotactic protein–3; IL-8, interleukin 8.
Human neutrophils were added to the upper compartment of the microchamber either simultaneously with or after 10 minutes of preincubation with different concentrations of chemokines. Results are expressed as mean CIs ± SEM. The figures in parentheses indicate the number of experiments.
When added to the lower compartment of the microchamber, IL-8 at 15 or 50 ng/mL induced the expected chemotactic migration, yielding CIs of at least 30.