Table 1. Antigen expression on NK1.1... | American Society of Hematology

Table 1.

Antigen expression on NK1.1⁺ thymocytes

Antigen	NK1.1⁺ CD3⁻ cells in B6	NK1.1⁺ CD3⁺ cells in ZAP-70^+/−	NK1.1⁺ CD3⁻ cells in ZAP-70^−/−	NK1.1⁺ CD3⁻ cells in Rag^−/−
T cell markers
TCRαβ	−	+	−	−
CD1	−	low	low	ND
CD4	−	+ (50%)	−	ND
CD5	−	+	−	−
CD8	−	−	−	−
CD24	−	−	−	ND
CD25	−	−	−	ND
CD44	+	+	++	++
CD90	−/+	+	+	+
CD122	+	+	+	+
NK cell markers
NKRP-1C	+	+	+	+
CD2	+	+	+	+
CD16	−/low	−/low	int	int
CD45R	−	−	int	int
CD45RB	+/low	−	+	+
CD62L	−	−	+/−	+/−
CD69	−/low	−/low	int	int
CD95	+	+	−/low	−/low
Ly49A	+ (10%)	+ (20%)	−	−
Ly49C	+ (25%)	+ (60%)	−	−
2B4^*	−	−	+	+
DX5	+	−^†	+	+
Stem cell markers
CD34	−	−	−	ND
CD117	−	−	−	ND

Antigen	NK1.1⁺ CD3⁻ cells in B6	NK1.1⁺ CD3⁺ cells in ZAP-70^+/−	NK1.1⁺ CD3⁻ cells in ZAP-70^−/−	NK1.1⁺ CD3⁻ cells in Rag^−/−
T cell markers
TCRαβ	−	+	−	−
CD1	−	low	low	ND
CD4	−	+ (50%)	−	ND
CD5	−	+	−	−
CD8	−	−	−	−
CD24	−	−	−	ND
CD25	−	−	−	ND
CD44	+	+	++	++
CD90	−/+	+	+	+
CD122	+	+	+	+
NK cell markers
NKRP-1C	+	+	+	+
CD2	+	+	+	+
CD16	−/low	−/low	int	int
CD45R	−	−	int	int
CD45RB	+/low	−	+	+
CD62L	−	−	+/−	+/−
CD69	−/low	−/low	int	int
CD95	+	+	−/low	−/low
Ly49A	+ (10%)	+ (20%)	−	−
Ly49C	+ (25%)	+ (60%)	−	−
2B4^*	−	−	+	+
DX5	+	−^†	+	+
Stem cell markers
CD34	−	−	−	ND
CD117	−	−	−	ND

Numbers in parentheses indicate the proportions of cells that express respective antigens; ND indicates not determined.

The expression of 2B4 is not observed on freshly isolated NK cells. However, 2B4 is expressed on cultured NK cells.47

Thirty percent of the cells were positive in spleen.