Peripheral blood and skin biopsy specimens were obtained after individual consent and according to the provisions of the City of Hope Institutional Review Board. Individuals were first screened by known HLA type (per the City of Hope or National Marrow Donor program) and were checked for CMV seropositivity by indirect immunofluorescence (Hemagen Diagnostic, Columbia, MD) at the City of Hope Clinical Microbiology Laboratory. Both HLA alleles are shown for each individual as determined by serologic analysis using microcytotoxicity assay. TCCs were isolated from IVS cultures of selected donors, and theTCR gene structure was identified by PCR and DNA sequencing as described in “Materials and methods.”

HLA indicates human leukocyte antigen; TCC, T-cell clone; TCR, T-cell receptor; CMV, cytomegalovirus; ND not determined; PCR, polymerase chain reaction.