Primers used in the reverse transcriptase–polymerase chain reaction analysis
| Gene . | 5′ Primer . | 3′ Primer . |
|---|---|---|
| Human C/EBP-α | 5′-GAATCTCCTAGTCCTGGCTC | 5′-GATGAGAACAGCAACGAGTAC |
| Mouse C/EBP-γ | 5′-CAGAGAGCGGAACAATATGG | 5′-TCAGCTGTTCAGTGTTGAGC |
| Mouse C/EBP-δ | 5′-AGAACGAGAAGCTGCATCAG | 5′-GTCTGAGGTATAGGTCGTTC |
| Human C/EBP-ε | 5′-CAGACAGGAAGGCGCTGGG | 5′-CGGCAGTGGCCAAAGGGGCCTT |
| Mouse GM-CSFR | 5′-CTGATGTCATGAAGCGATGC | 5′-TCACGGTGACATCAATGTCG |
| Mouse G-CSFR | 5′-AAACCTTCCAGTTACCCAGC | 5′-TGGAGTCAGAGCGAATGTAC |
| Mouse M-CSFR | 5′-AACATATGGACTTCGCCCTC | 5′-ACCTTCCAGTCTAGAGTGAC |
| Mouse MPO | 5′-GTCCAGATCATCACATACCG | 5′-CCTATCCTCATGACTTGCTC |
| Mouse CD18 | 5′-TTCAGTGTGCTGGTATGACG | 5′-ACACCAATCAGTACGACACC |
| Mouse neutrophil elastase | 5′-CATGCTACTGGCATTGTTCC | 5′-TCGTTCAGCAGTTGTGATGG |
| Mouse HLS7 | 5′-TGGCTGTTGGTCATCACATC | 5′-TCTTCCACTCTCAATGGCTG |
| Mouse MLL (ALL-1) | 5′-ATTCCTCTTTCAGCTCAGCC | 5′-GGTTACACAGTTGTCACTGC |
| Mouse CD19 | 5′-AATCTGGAGATCCCAGATGG | 5′-TAGCGGCTGGACATATTTGC |
| Mouse Pax-5 | 5′-AGCATAGTGTCTACAGGCTC | 5′-TGGCTTTCATGTCATCCAGG |
| Mouse Igκ chain | 5′-CAAAATGGCGTCCTGAACAG | 5′-TTGATGTCTTGTGAGTGGCC |
| Mouse Igμ chain | 5′-CTGGTCTCAAACCTGGCAAC | 5′-CTGTCACAGTCAGGATGCTG |
| Mouse β-actin | 5′-CACCCTGTGCTGCTCACCGAGGCC | 5′-CCACACAGATGACTTGCGCTCAGG |
| Gene . | 5′ Primer . | 3′ Primer . |
|---|---|---|
| Human C/EBP-α | 5′-GAATCTCCTAGTCCTGGCTC | 5′-GATGAGAACAGCAACGAGTAC |
| Mouse C/EBP-γ | 5′-CAGAGAGCGGAACAATATGG | 5′-TCAGCTGTTCAGTGTTGAGC |
| Mouse C/EBP-δ | 5′-AGAACGAGAAGCTGCATCAG | 5′-GTCTGAGGTATAGGTCGTTC |
| Human C/EBP-ε | 5′-CAGACAGGAAGGCGCTGGG | 5′-CGGCAGTGGCCAAAGGGGCCTT |
| Mouse GM-CSFR | 5′-CTGATGTCATGAAGCGATGC | 5′-TCACGGTGACATCAATGTCG |
| Mouse G-CSFR | 5′-AAACCTTCCAGTTACCCAGC | 5′-TGGAGTCAGAGCGAATGTAC |
| Mouse M-CSFR | 5′-AACATATGGACTTCGCCCTC | 5′-ACCTTCCAGTCTAGAGTGAC |
| Mouse MPO | 5′-GTCCAGATCATCACATACCG | 5′-CCTATCCTCATGACTTGCTC |
| Mouse CD18 | 5′-TTCAGTGTGCTGGTATGACG | 5′-ACACCAATCAGTACGACACC |
| Mouse neutrophil elastase | 5′-CATGCTACTGGCATTGTTCC | 5′-TCGTTCAGCAGTTGTGATGG |
| Mouse HLS7 | 5′-TGGCTGTTGGTCATCACATC | 5′-TCTTCCACTCTCAATGGCTG |
| Mouse MLL (ALL-1) | 5′-ATTCCTCTTTCAGCTCAGCC | 5′-GGTTACACAGTTGTCACTGC |
| Mouse CD19 | 5′-AATCTGGAGATCCCAGATGG | 5′-TAGCGGCTGGACATATTTGC |
| Mouse Pax-5 | 5′-AGCATAGTGTCTACAGGCTC | 5′-TGGCTTTCATGTCATCCAGG |
| Mouse Igκ chain | 5′-CAAAATGGCGTCCTGAACAG | 5′-TTGATGTCTTGTGAGTGGCC |
| Mouse Igμ chain | 5′-CTGGTCTCAAACCTGGCAAC | 5′-CTGTCACAGTCAGGATGCTG |
| Mouse β-actin | 5′-CACCCTGTGCTGCTCACCGAGGCC | 5′-CCACACAGATGACTTGCGCTCAGG |
C/EBP indicates CAAT-enhancer-binding protein; GM-CSFR, granulocyte-macrophage colony-stimulating factor receptor; G-CSFR, granulocyte colony-stimulating factor receptor; M-CSFR, macrophage colony-stimulating factor receptor; MPO, myeloperoxidase; HLS7, hematopoietic lineage switch 7; MLL, mixed lineage leukemia; ALL, acute lymphoblastic leukemia; Ig, immunoglobulin.