Quantitative evaluation of apoptosis, performed by flow cytometry 24 hours after treatment with ionizing radiation alone or in combination with (6 hours before) tumor necrosis factor–related apoptosis-inducing ligand (1 μg/mL)
| . | HEL . | K562 . | Friend . | Erythroleukemic blasts . | Normal erythroblasts . | |
|---|---|---|---|---|---|---|
| No. 1 . | No. 2 . | |||||
| Control | 4 ± 2.0 | 6 ± 2.0 | 1 ± 0.5 | 10 | 14 | 14 ± 5.0 |
| 1.5 Gy | 5 ± 1.8 | 5 ± 2.0 | 2 ± 1.0 | 15 | 19 | 25 ± 5.5 |
| 15 Gy | 6 ± 2.0 | 6 ± 2.5 | 3 ± 1.5 | 17 | 18 | 28 ± 6.0 |
| TRAIL | 18 ± 2.0 | 12 ± 3.0 | 3 ± 1.0 | 17 | 20 | 27 ± 3.5 |
| 1.5 Gy before TRAIL | 45 ± 5.0 | 17 ± 3.0 | 10 ± 3.0 | 28 | 31 | 29 ± 5.0 |
| 15 Gy before TRAIL | 51 ± 5.0 | 20 ± 5.0 | 12 ± 4.0 | 31 | 38 | 30 ± 6.5 |
| . | HEL . | K562 . | Friend . | Erythroleukemic blasts . | Normal erythroblasts . | |
|---|---|---|---|---|---|---|
| No. 1 . | No. 2 . | |||||
| Control | 4 ± 2.0 | 6 ± 2.0 | 1 ± 0.5 | 10 | 14 | 14 ± 5.0 |
| 1.5 Gy | 5 ± 1.8 | 5 ± 2.0 | 2 ± 1.0 | 15 | 19 | 25 ± 5.5 |
| 15 Gy | 6 ± 2.0 | 6 ± 2.5 | 3 ± 1.5 | 17 | 18 | 28 ± 6.0 |
| TRAIL | 18 ± 2.0 | 12 ± 3.0 | 3 ± 1.0 | 17 | 20 | 27 ± 3.5 |
| 1.5 Gy before TRAIL | 45 ± 5.0 | 17 ± 3.0 | 10 ± 3.0 | 28 | 31 | 29 ± 5.0 |
| 15 Gy before TRAIL | 51 ± 5.0 | 20 ± 5.0 | 12 ± 4.0 | 31 | 38 | 30 ± 6.5 |
Data represent the mean ± SD of 4 (HEL, K562, and Friend cell lines) or 5 (normal erythroblasts) separate experiments, performed in duplicate. Data of experiments performed in duplicate with erythroleukemic blasts obtained from 2 different patients (nos. 1 and 2) are reported individually.
TRAIL indicates tumor necrosis factor–related apoptosis-inducing ligand.