Binding characteristics of different cysteine-rich interdomain region 1 to CD36
| Parasite phenotype . | Construct expressed . | Positive cells (%) . |
|---|---|---|
| CD36+/CSA− | MC CIDR-1 | 99 ± 1 |
| A4 CIDR-1 | 97 ± 1 | |
| A4 tres CIDR-1 | 89 ± 3 | |
| FVO CIDR-1 | 93 ± 2 | |
| MC M2 | 92 ± 5.5 | |
| FVO M2 | 46 ± 7.5 | |
| CD36−/CSA+ | FCR3-CSA CIDR-1 | 1 ± 1 |
| ItG2-CS2 CIDR-1 | 1 ± 1 | |
| Unknown | 3D7AL010226 | 0 ± 0 |
| Parasite phenotype . | Construct expressed . | Positive cells (%) . |
|---|---|---|
| CD36+/CSA− | MC CIDR-1 | 99 ± 1 |
| A4 CIDR-1 | 97 ± 1 | |
| A4 tres CIDR-1 | 89 ± 3 | |
| FVO CIDR-1 | 93 ± 2 | |
| MC M2 | 92 ± 5.5 | |
| FVO M2 | 46 ± 7.5 | |
| CD36−/CSA+ | FCR3-CSA CIDR-1 | 1 ± 1 |
| ItG2-CS2 CIDR-1 | 1 ± 1 | |
| Unknown | 3D7AL010226 | 0 ± 0 |
CIDR-1 and M2 constructs from CD36 (CD36+/CSA1−) and CSA (CD36−/CSA+) adherent parasites were transfected into COS-7 cells and tested for binding to CD36-coated Dynal beads.
One hundred cells expressing the recombinant protein, determined by immunofluorescence, were checked for the presence of CD36-coated Dynal beads on their surface. Cells with 4 or more beads attached were considered positive for binding. Results are expressed as the mean and SD of 3 independent experiments.
MC indicates Malayan Camp; CIDR-1, cysteine-rich interdomain region 1.