Early lymphocyte precursors are direct targets for negative regulation by hormones and cytokines
| Factor (concentration) . | CD45R+, CD19−cells . | CD45R+, CD19+ cells . |
|---|---|---|
| β-Estradiol (10−8 M)* | 239.8 ± 208.7 | 22.0 ± 11.0 |
| Limitin (200 ng/mL)† | 5.6, 4.7 | 1.2, 0.1 |
| Interferon-α (10 U/mL)† | 14.9, 3.3 | 5.9, 4.2 |
| Interferon-γ (10 U/mL)† | 19.5, 17.8 | <0.1, 0.3 |
| TGF-β (1 ng/mL)† | 8.3, 9.0 | 11.0, 9.1 |
| EGF (50 ng/mL)† | 94.1, 62.9 | 82.2, 87.2 |
| Factor (concentration) . | CD45R+, CD19−cells . | CD45R+, CD19+ cells . |
|---|---|---|
| β-Estradiol (10−8 M)* | 239.8 ± 208.7 | 22.0 ± 11.0 |
| Limitin (200 ng/mL)† | 5.6, 4.7 | 1.2, 0.1 |
| Interferon-α (10 U/mL)† | 14.9, 3.3 | 5.9, 4.2 |
| Interferon-γ (10 U/mL)† | 19.5, 17.8 | <0.1, 0.3 |
| TGF-β (1 ng/mL)† | 8.3, 9.0 | 11.0, 9.1 |
| EGF (50 ng/mL)† | 94.1, 62.9 | 82.2, 87.2 |
TGF-β indicates transforming growth factor β; EGF, epidermal growth factor.
Highly purified Lin− c-kitLo cells were held for 1 week in serum-free, stromal cell-free cultures. Yields of CD45R+CD19+ and CD45R+CD19− cells per input precursor were calculated and then normalized as percentages of control values. These data represent means ± SD from 5 independent experiments.
Results from each of two experiments are separated by commas.