Patient characteristics and culture data
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FAB indicates French-American-British; MLR, mixed lymphocyte reaction; GM, granulocyte-macrophage colony stimulating factor; IL, interleukin.
Cytokine combination giving best combination of morphological, phenotypic, and functional maturation for each sample. T0 = tumor necrosis factor α [TNF-α] from day 0, T(2d) = TNF-α added for the last 2 days of culture. Equivalent indicates no cytokine combination better than any other.
− Mature cell density < 0.25 × 106 cells/mL; + mature cells 0.25-0.50 × 106/mL; ++ mature cells 0.50-0.75 × 106/mL; +++ mature cells > 0.75 × 106/mL. (Initial blast cell density = 1 × 106/mL).
Circles represent expression of HLA-DR, CD86, CD1a, CD83, and CD14, in that order, at the beginning and end of culture, after subtraction of controls, on the following: ○ <10%; 10%-25%; ◐ 26%-50%;51%-75%; ● >75% of cells.
Numbers represent fold greater proliferation of responders in presence of allogeneic or autologous stimulators than in presence of controls (responder to stimulator = 4:1).