Effect of varying culture conditions on growth and maturation of dendritic cells derived from acute myeloid leukemia blasts
| Comparison . | No. experiments . | Density of viable cells at end of culture (×106/mL) . | Density of mature cells* at end of culture (×106/mL) . | ||
|---|---|---|---|---|---|
| Mean ± SD . | P value (Student t test) . | Mean ± SD . | P value (Student t test) . | ||
| Length of culture | |||||
| ≤9 days | 33 | 1.23 ± 0.71 | P = .59 | 0.66 ± 0.41 | P = .14 |
| >9 days | 7 | 1.05 ± 0.79 | 0.45 ± 0.30 | ||
| Sera | |||||
| FCS versus AB/autologous | 4 | ||||
| FCS | 1.54 ± 1.53 | P = .37† | 0.45 ± 0.31 | P = .71† | |
| AB/autologous | 0.91 ± 0.44 | 0.37 ± 0.24 | |||
| AB versus autologous | 4 | ||||
| AB | 1.22 ± 0.40 | P = .24† | 0.52 ± 0.23 | P = .42† | |
| Autologous | 0.90 ± 0.52 | 0.39 ± 0.26 | |||
| Fresh/thawed samples‡ | |||||
| Fresh | 6 | 1.28 ± 0.32 | P = .10 | 0.75 ± 0.21 | P = .13 |
| Cryopreserved | 20 | 0.94 ± 0.68 | 0.54 ± 0.42 | ||
| FAB type | |||||
| M0/M1/M2 | 26 | 1.30 ± 0.74 | P = .17 | 0.66 ± 0.44 | P = .66 |
| M4/M5 | 12 | 0.96 ± 0.61 | 0.59 ± 0.46 | ||
| Cytokine combinations | |||||
| GM-CSF ± TNF-α | 38 | ||||
| with IL-4 | 0.80 ± 0.60 | P = .29 | 0.42 ± 0.38 | P = .35 | |
| without IL-4 | 0.89 ± 0.64 | 0.37 ± 0.36 | |||
| GM-CSF ± IL-4 | 40 | ||||
| with TNF-α d0/last 2d | 0.86 ± 0.64 | P = .50 | 0.43 ± 0.39 | P < .01 | |
| without TNF-α | 0.80 ± 0.55 | 0.29 ± 0.25 | |||
| GM-CSF ± IL-4 | 39 | ||||
| with TNF-α d0 | 0.88 ± 0.68 | P = .70 | 0.48 ± 0.43 | P = .10 | |
| with TNF-α last 2d | 0.84 ± 0.60 | 0.38 ± 0.34 | |||
| FAB types M4/M5 only | |||||
| GM-CSF, TNF-α d0 | 12 | 0.69 ± 0.56 | P = .08 | 0.32 ± 0.31 | P < .05 |
| GM-CSF, IL4, TNF-α d0 | 12 | 0.91 ± 0.55 | 0.52 ± 0.42 | ||
| Comparison . | No. experiments . | Density of viable cells at end of culture (×106/mL) . | Density of mature cells* at end of culture (×106/mL) . | ||
|---|---|---|---|---|---|
| Mean ± SD . | P value (Student t test) . | Mean ± SD . | P value (Student t test) . | ||
| Length of culture | |||||
| ≤9 days | 33 | 1.23 ± 0.71 | P = .59 | 0.66 ± 0.41 | P = .14 |
| >9 days | 7 | 1.05 ± 0.79 | 0.45 ± 0.30 | ||
| Sera | |||||
| FCS versus AB/autologous | 4 | ||||
| FCS | 1.54 ± 1.53 | P = .37† | 0.45 ± 0.31 | P = .71† | |
| AB/autologous | 0.91 ± 0.44 | 0.37 ± 0.24 | |||
| AB versus autologous | 4 | ||||
| AB | 1.22 ± 0.40 | P = .24† | 0.52 ± 0.23 | P = .42† | |
| Autologous | 0.90 ± 0.52 | 0.39 ± 0.26 | |||
| Fresh/thawed samples‡ | |||||
| Fresh | 6 | 1.28 ± 0.32 | P = .10 | 0.75 ± 0.21 | P = .13 |
| Cryopreserved | 20 | 0.94 ± 0.68 | 0.54 ± 0.42 | ||
| FAB type | |||||
| M0/M1/M2 | 26 | 1.30 ± 0.74 | P = .17 | 0.66 ± 0.44 | P = .66 |
| M4/M5 | 12 | 0.96 ± 0.61 | 0.59 ± 0.46 | ||
| Cytokine combinations | |||||
| GM-CSF ± TNF-α | 38 | ||||
| with IL-4 | 0.80 ± 0.60 | P = .29 | 0.42 ± 0.38 | P = .35 | |
| without IL-4 | 0.89 ± 0.64 | 0.37 ± 0.36 | |||
| GM-CSF ± IL-4 | 40 | ||||
| with TNF-α d0/last 2d | 0.86 ± 0.64 | P = .50 | 0.43 ± 0.39 | P < .01 | |
| without TNF-α | 0.80 ± 0.55 | 0.29 ± 0.25 | |||
| GM-CSF ± IL-4 | 39 | ||||
| with TNF-α d0 | 0.88 ± 0.68 | P = .70 | 0.48 ± 0.43 | P = .10 | |
| with TNF-α last 2d | 0.84 ± 0.60 | 0.38 ± 0.34 | |||
| FAB types M4/M5 only | |||||
| GM-CSF, TNF-α d0 | 12 | 0.69 ± 0.56 | P = .08 | 0.32 ± 0.31 | P < .05 |
| GM-CSF, IL4, TNF-α d0 | 12 | 0.91 ± 0.55 | 0.52 ± 0.42 | ||
All cell-density numbers are cell densities after culture; initial blast cell density = 1 × 106cells/mL.
FCS indicates fetal calf serum; FAB, French-American-British; AB, blood group AB serum; TNF, tumor necrosis factor; GM-CSF, granulocyte-macrophage colony stimulating factor; IL, interleukin.
Mature cells are cells defined as mature leukemic dendritic cells morphologically.
As multiple data were available for these experiments, they were analyzed by a repeated-measures analysis of variance (SPSS, statistical software package).
Only samples cultured for 7 to 8 days in AB serum were compared.