Effects of stromal cell–derived factor 1α on proplatelet formation
| Experiment no. . | Growth factor . | Proplatelet-bearing MKs, % . |
|---|---|---|
| 1 | TPO | 29 |
| TPO + SDF-1α | 48 | |
| 2 | TPO | 24 |
| TPO + SDF-1α | 55 | |
| 3 | TPO | 19 |
| TPO + SDF-1α | 41 | |
| Mean ± SEM | TPO | 24 ± 2.8 |
| TPO + SDF-1α | 48 ± 4* |
| Experiment no. . | Growth factor . | Proplatelet-bearing MKs, % . |
|---|---|---|
| 1 | TPO | 29 |
| TPO + SDF-1α | 48 | |
| 2 | TPO | 24 |
| TPO + SDF-1α | 55 | |
| 3 | TPO | 19 |
| TPO + SDF-1α | 41 | |
| Mean ± SEM | TPO | 24 ± 2.8 |
| TPO + SDF-1α | 48 ± 4* |
Data refer to HPC-derived MKs grown in serum-free liquid culture at day 12. TPO (100 ng/mL) was used with and without SDF-1α (1 μg/mL).
SDF-1α indicates stromal cell–derived factor 1α; TPO, thrombopoietin; MKs, megakaryocytes; HPC, hematopoietic progenitor cell.
P < .05 (Student t test) compared with cultures containing TPO alone.