Table 2. Effect of graded amounts of... | American Society of Hematology

Table 2.

Effect of graded amounts of thrombopoietin in the presence or absence of stromal cell–derived factor 1α (1 μg/mL) on megakaryocyte percentage, proliferation, and polyploidization

TPO	MKs, %		No. MKs × 10⁴/mL		Polylobated MKs, %
TPO	−SDF-1α	+SDF-1α	−SDF-1α	+SDF-1α	−SDF-1α	+SDF-1α
1 ng/mL	99	99	31.2 ± 10	30.3 ± 9	23 ± 7	50 ± 7^*
10 ng/mL	99	99	43.7 ± 8	38.5 ± 8	28 ± 5	54 ± 6^*
100 ng/mL	99	99	56.4 ± 11	55.7 ± 10	51 ± 2	73 ± 3^*

TPO	MKs, %		No. MKs × 10⁴/mL		Polylobated MKs, %
TPO	−SDF-1α	+SDF-1α	−SDF-1α	+SDF-1α	−SDF-1α	+SDF-1α
1 ng/mL	99	99	31.2 ± 10	30.3 ± 9	23 ± 7	50 ± 7^*
10 ng/mL	99	99	43.7 ± 8	38.5 ± 8	28 ± 5	54 ± 6^*
100 ng/mL	99	99	56.4 ± 11	55.7 ± 10	51 ± 2	73 ± 3^*

Data refer to hematopoietic progenitor cells grown in serum-free liquid culture at day 9. Based on mean ± SEM from 5 separate experiments.

For abbreviations, see Table 1.

P < .05 when compared with −SDF-1α by Studentt test.

View Large

This Feature Is Available To Subscribers Only