Activities of activated proteases in chromogenic substrate assays
| . | S-2366 . | Factor IX . | ||||
|---|---|---|---|---|---|---|
| Km (μM) . | kcat(sec−1) . | kcat/Km(sec−1 · μM−1) . | Km(μM) . | kcat(min−1) . | kcat/Km(min−1 · μM−1) . | |
| FXIa | 500 | 310 | 0.42 | 0.20 | 5.8 | 29 |
| FXIa/PKA4 | 450 | 250 | 0.56 | 0.12 | 3.4 | 28 |
| FXIa/PKA4-Gly326 | 590 | 430 | 0.72 | 0.16 | 5.7 | 36 |
| . | S-2366 . | Factor IX . | ||||
|---|---|---|---|---|---|---|
| Km (μM) . | kcat(sec−1) . | kcat/Km(sec−1 · μM−1) . | Km(μM) . | kcat(min−1) . | kcat/Km(min−1 · μM−1) . | |
| FXIa | 500 | 310 | 0.42 | 0.20 | 5.8 | 29 |
| FXIa/PKA4 | 450 | 250 | 0.56 | 0.12 | 3.4 | 28 |
| FXIa/PKA4-Gly326 | 590 | 430 | 0.72 | 0.16 | 5.7 | 36 |
Recombinant activated proteins were tested as described in “Materials and methods.” Results represent the averages of duplicate experiments.
Km indicates Michaelis-Menten constants; kcat, turnover number; FXIa, activated factor XI; FXIa/PKA4, monomeric version of FXIa; FXIa/PKA4-Gly326, dimeric version of FXIa/PKA4.