Differences in renal pathogenic activity between L8D and 6-19J monoclonal antibodies
| Antibody* . | IgG3* . | Cryoglobulin* . | Kidney lesions† . |
|---|---|---|---|
| L8D | 3.8 ± 1.6 | 14.6 ± 6.8 | 8/8 |
| 6-19J | 3.4 ± 2.1 | 4.1 ± 2.7 | 0/17 |
| Antibody* . | IgG3* . | Cryoglobulin* . | Kidney lesions† . |
|---|---|---|---|
| L8D | 3.8 ± 1.6 | 14.6 ± 6.8 | 8/8 |
| 6-19J | 3.4 ± 2.1 | 4.1 ± 2.7 | 0/17 |
IgG indicates immunoglobulin G.
107 cells secreting L8D or 6-19J IgG3 mAb were inoculated intraperitoneally into (MRL × BALB/c)F1 mice. L8D- and 6-19J–injected mice were killed 8 to 10 days and approximately 3 weeks later, respectively, for serologic and histologic analysis. Serum levels of IgG3 (mg/mL) and cryoglobulins (μg/mL), measured at euthanasia, are shown (means ± 1 SD). Serum levels of IgG3 and cryoglobulins before the injection of hybridoma cells were 0.4 ± 0.2 mg/mL and < 0.1 μg/mL, respectively.
Incidence of glomerulonephritis. When the severity of glomerular lesions was semiquantitatively evaluated on a 0 to 4+ scale, mice injected with L8D cells developed very severe lesions, the mean was 3.1 ± 0.5. In contrast, none of the mice injected with 6-19J cells developed significant glomerular lesions.