Leukemic cells produce IFN-α when stimulated with influenza virus
| Patients . | IFN-α (U/mL) . | |||||
|---|---|---|---|---|---|---|
| Enriched tumor cells . | Sorted cells . | |||||
| CD56+ . | CD56− . | |||||
| Medium . | Influenza virus . | Medium . | Influenza virus . | Medium . | Influenza virus . | |
| CAD (bone marrow) | < 0.6 | 3 | nd | nd | nd | nd |
| LAI (bone marrow) | nd | nd | < 0.6 | 30 | < 0.6 | < 0.6 |
| GEN (blood) | ||||||
| Experiment 1 | 9 | 1302 | nd | nd | nd | nd |
| Experiment 2 | < 0.6 | 1695 | < 0.6 | 912 | < 0.6 | 2026 |
| GUE (lymph node) | ||||||
| Experiment 1 | 6 | 1099 | nd | nd | nd | nd |
| Experiment 2 | nd | nd | < 0.6 | 1146 | < 0.6 | 1235 |
| Patients . | IFN-α (U/mL) . | |||||
|---|---|---|---|---|---|---|
| Enriched tumor cells . | Sorted cells . | |||||
| CD56+ . | CD56− . | |||||
| Medium . | Influenza virus . | Medium . | Influenza virus . | Medium . | Influenza virus . | |
| CAD (bone marrow) | < 0.6 | 3 | nd | nd | nd | nd |
| LAI (bone marrow) | nd | nd | < 0.6 | 30 | < 0.6 | < 0.6 |
| GEN (blood) | ||||||
| Experiment 1 | 9 | 1302 | nd | nd | nd | nd |
| Experiment 2 | < 0.6 | 1695 | < 0.6 | 912 | < 0.6 | 2026 |
| GUE (lymph node) | ||||||
| Experiment 1 | 6 | 1099 | nd | nd | nd | nd |
| Experiment 2 | nd | nd | < 0.6 | 1146 | < 0.6 | 1235 |
The production of IFN-α by enriched and sorted tumor cells was measured by ELISA after a 24-hour stimulation with influenza virus. In parallel, sorted CD11c− pDCs and CD11c+ DCs were found to produce 24 990 and 57 IU/mL IFN-α, respectively, in response to virus, while in the absence of virus the values were below 0.6 IU/mL.
nd indicates not done.