Table 1. Location of cytomegalovirus in... | American Society of Hematology

Table 1.

Location of cytomegalovirus in white blood cell subsets

Subject	Peripheral blood		FACS analysis (sample 2)
	GE/mL	GE/mL	FACS analysis (sample 2)
	Sample 1	Sample 2	CD14	CD4	CD3	CD19	GRAN
1	2 100	—	POS	—	—	—	—
2	25 200	4 800	POS	—	—	—	—
3	—	24 000	POS	—	—	—	POS
4	—	—	—	—	—	—	—
5	—	—	—	—	—	—	—
6	43 600	—	POS	—	—	—	—
7	17 400	—	POS	—	—	—	—
8	15 800	—	POS	—	—	—	—
9	—	—	—	—	—	—	—
10	2 600	—	POS	—	—	—	—
11	—	—	—	—	—	—	—
12	—	—	POS	—	—	—	—
13	300	—	—	—	—	—	—
14	3 100	—	POS	—	—	—	—
15	1 000 000	720 000	POS	—	—	—	POS
16	—	—	—	—	—	—	—
17	—	—	POS	—	—	—	—
18	—	—	—	—	—	—	—
19	—	—	POS	—	—	—	—
20	3 800	—	POS	—	—	—	POS

Subject	Peripheral blood		FACS analysis (sample 2)
	GE/mL	GE/mL	FACS analysis (sample 2)
	Sample 1	Sample 2	CD14	CD4	CD3	CD19	GRAN
1	2 100	—	POS	—	—	—	—
2	25 200	4 800	POS	—	—	—	—
3	—	24 000	POS	—	—	—	POS
4	—	—	—	—	—	—	—
5	—	—	—	—	—	—	—
6	43 600	—	POS	—	—	—	—
7	17 400	—	POS	—	—	—	—
8	15 800	—	POS	—	—	—	—
9	—	—	—	—	—	—	—
10	2 600	—	POS	—	—	—	—
11	—	—	—	—	—	—	—
12	—	—	POS	—	—	—	—
13	300	—	—	—	—	—	—
14	3 100	—	POS	—	—	—	—
15	1 000 000	720 000	POS	—	—	—	POS
16	—	—	—	—	—	—	—
17	—	—	POS	—	—	—	—
18	—	—	—	—	—	—	—
19	—	—	POS	—	—	—	—
20	3 800	—	POS	—	—	—	POS

From the spring 1998 cohort, 10 DNAemia positive and 10 negative subjects were identified from the initial screening (sample 1) of peripheral blood samples (limit of detection 100 GE/mL). During a subsequent visit, a second sample (sample 2) was obtained. White blood cells (WBCs) were isolated and sorted by surface antigen phenotype. Isolated subpopulations were then assayed by QA-PCR. The CMV was detected in monocytes (CD14) in 13 of 20 samples, even for those samples that had a titer of less than or equal to 100 GE/mL. Cytomegalovirus (CMV) was detected in the granulocyte fraction for 3 subjects.

GE indicates genome equivalents; FACS, fluorescence-activated cell sorter; POS, positive; GRAN, granulocyte.

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