Sensitivity for Rapa-induced apoptosis and cell cycle arrest of different cell types
| Cell types . | Apoptosis, % . | No. of experiments . | G0/G1 fraction, % . | No. of experiments . | |||
|---|---|---|---|---|---|---|---|
| Spontaneous . | Rapa . | Etoposide . | Control . | Rapa . | |||
| Monocytes | 4.3 | 3.5 | 3.8 | 5 | ND | ND | 0 |
| Macrophages (monocyte-derived) | 2.9 | 0 | 0.7 | 4 | ND | ND | 0 |
| Alveolar macrophages | 3.0 | 2.4 | 2.0 | 2 | ND | ND | 0 |
| Dendritic cells (monocyte-derived) | 5.9 | 45.2* | 6.3 | 6 | 100.0 | 100.0 | 2 |
| Dendritic cells (CD34-derived) | 6.8 | 21.5* | ND | 4 | ND | ND | 0 |
| U937 | 2.0 | 2.3 | 74.9* | 3 | 61.0 | 61.1 | 3 |
| THP-1 | 3.9 | 4.8 | 86.2* | 2 | 52.8 | 53.5 | 1 |
| MonoMac6 | 11.5 | 11.0 | 48.1* | 3 | 61.1 | 67.7 | 3 |
| KG-1 | 8.6 | 5.5 | 22.7* | 5 | 53.5 | 50.7 | 4 |
| Jurkat | 2.3 | 2.4 | 71.2* | 3 | 48.7 | 70.8* | 3 |
| Cell types . | Apoptosis, % . | No. of experiments . | G0/G1 fraction, % . | No. of experiments . | |||
|---|---|---|---|---|---|---|---|
| Spontaneous . | Rapa . | Etoposide . | Control . | Rapa . | |||
| Monocytes | 4.3 | 3.5 | 3.8 | 5 | ND | ND | 0 |
| Macrophages (monocyte-derived) | 2.9 | 0 | 0.7 | 4 | ND | ND | 0 |
| Alveolar macrophages | 3.0 | 2.4 | 2.0 | 2 | ND | ND | 0 |
| Dendritic cells (monocyte-derived) | 5.9 | 45.2* | 6.3 | 6 | 100.0 | 100.0 | 2 |
| Dendritic cells (CD34-derived) | 6.8 | 21.5* | ND | 4 | ND | ND | 0 |
| U937 | 2.0 | 2.3 | 74.9* | 3 | 61.0 | 61.1 | 3 |
| THP-1 | 3.9 | 4.8 | 86.2* | 2 | 52.8 | 53.5 | 1 |
| MonoMac6 | 11.5 | 11.0 | 48.1* | 3 | 61.1 | 67.7 | 3 |
| KG-1 | 8.6 | 5.5 | 22.7* | 5 | 53.5 | 50.7 | 4 |
| Jurkat | 2.3 | 2.4 | 71.2* | 3 | 48.7 | 70.8* | 3 |
The different cell types were cultured as described in “Materials and methods.” Cells were cultured with or without 10−7 M Rapa or 10−5 M etoposide. Except the CD34+-derived DCs, which were analyzed after 24 hours, cells were harvested after 48 hours and analyzed for apoptosis using annexin V/PI staining and Hoechst or cell cycle progression using DNA staining. Cell cycle analysis for the percentage of cells present in G0/G1 was performed by excluding cells containing fewer than 2N DNA.
ND indicates not determined.
Significant differences induced by Rapa/etoposide (P < .05, Student t test for paired samples).